Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OSC48_RS25075 Genome accession   NZ_CP112867
Coordinates   5433552..5434586 (-) Length   344 a.a.
NCBI ID   WP_253509705.1    Uniprot ID   -
Organism   Pseudomonas quebecensis isolate S1Bt7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5428552..5439586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSC48_RS25045 (OSC48_25045) ruvX 5428781..5429218 (+) 438 WP_003176676.1 Holliday junction resolvase RuvX -
  OSC48_RS25050 (OSC48_25050) pyrR 5429301..5429807 (+) 507 WP_266247258.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  OSC48_RS25055 (OSC48_25055) - 5429832..5430836 (+) 1005 WP_181076535.1 aspartate carbamoyltransferase catalytic subunit -
  OSC48_RS25060 (OSC48_25060) - 5430833..5432104 (+) 1272 WP_181076534.1 dihydroorotase -
  OSC48_RS25065 (OSC48_25065) - 5432177..5432587 (-) 411 WP_181076588.1 TM2 domain-containing protein -
  OSC48_RS25070 (OSC48_25070) - 5432855..5433490 (+) 636 WP_181076532.1 C40 family peptidase -
  OSC48_RS25075 (OSC48_25075) pilT 5433552..5434586 (-) 1035 WP_253509705.1 type IV pilus twitching motility protein PilT Machinery gene
  OSC48_RS25080 (OSC48_25080) - 5434644..5435330 (+) 687 WP_181076529.1 YggS family pyridoxal phosphate-dependent enzyme -
  OSC48_RS25085 (OSC48_25085) proC 5435364..5436182 (+) 819 WP_266247260.1 pyrroline-5-carboxylate reductase -
  OSC48_RS25090 (OSC48_25090) - 5436207..5436794 (+) 588 WP_181076526.1 YggT family protein -
  OSC48_RS25095 (OSC48_25095) - 5436935..5438074 (+) 1140 WP_181076524.1 homoserine O-acetyltransferase -
  OSC48_RS25100 (OSC48_25100) metW 5438082..5438702 (+) 621 WP_181076522.1 methionine biosynthesis protein MetW -
  OSC48_RS25105 (OSC48_25105) - 5438724..5439158 (+) 435 WP_181076521.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37719.62 Da        Isoelectric Point: 7.0437

>NTDB_id=758489 OSC48_RS25075 WP_253509705.1 5433552..5434586(-) (pilT) [Pseudomonas quebecensis isolate S1Bt7]
MDIAQLLTVSVRRGASDLHLSAGLVPMLRIDGEVWPLDWPVLSARQVADLLSPLLNEHQQKDFETSLETDFSFELPDVAR
FRANVFQQDRGMGAVFRTIPTEVLSLERLGLGDVFQRIARLPRGLVLVTGPTGSGKSTTLAAMIDYLNQHRRQHILTLED
PIEFIHTPKVALINQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKLGGGRAAAHEIMLGTPAIRNLIREDKVAQMVSAMQTGGALGMKTLDMSL
KALVAQGVISREEAREKARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=758489 OSC48_RS25075 WP_253509705.1 5433552..5434586(-) (pilT) [Pseudomonas quebecensis isolate S1Bt7]
ATGGATATCGCTCAATTGCTGACGGTCAGCGTGCGCCGAGGCGCCTCCGACCTGCATCTCTCGGCAGGCCTGGTGCCAAT
GTTAAGGATCGATGGCGAGGTTTGGCCCCTGGATTGGCCCGTACTTTCAGCCCGGCAAGTTGCAGATTTGCTGAGCCCGT
TGCTTAACGAGCACCAACAAAAGGATTTCGAAACGTCTCTTGAAACTGATTTTTCCTTCGAGCTGCCCGATGTGGCACGG
TTCCGGGCTAACGTGTTCCAGCAGGATCGCGGCATGGGCGCAGTGTTTCGCACCATCCCGACGGAGGTCCTGAGTCTGGA
GAGGCTGGGCCTGGGCGATGTATTCCAACGTATCGCCCGGTTACCGCGCGGCCTGGTGCTGGTGACCGGGCCTACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCGATGATCGACTACCTCAACCAGCACCGGCGCCAGCACATCCTCACGTTGGAAGAC
CCCATCGAATTTATCCACACGCCGAAAGTCGCCCTGATCAATCAACGCCAGGTGCACCGTGATACGCACAGTTTTTCGGT
AGCGCTGCGCTCGGCATTGCGCGAAGACCCCGATGTAATCCTGGTCGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CATTGACCGCAGCGGAGACCGGGCACCTGGTGTTCGGCACCTTGCACACCACCTCGGCGGCGAAAACCATAGATCGCCTG
GTCGATGTGTTCCCCGCAGGCGAAAAGGCCATGGTGCGTTCGATGCTGTCGGAGTCGCTGCAAGCCGTGGTGTCCCAGGT
GCTGGTGAAGAAGCTCGGCGGCGGGCGTGCGGCGGCCCATGAAATCATGCTGGGCACACCGGCAATTCGTAATCTGATCC
GTGAAGACAAAGTGGCTCAGATGGTCTCGGCGATGCAGACGGGGGGCGCGCTGGGGATGAAGACACTGGATATGAGTTTG
AAGGCGCTGGTCGCGCAGGGCGTGATCAGCCGGGAAGAGGCGCGGGAAAAGGCGAGGGTGCCTGGGGATATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.607

99.128

0.73
  pilT Pseudomonas stutzeri DSM 10701

71.554

99.128

0.709
  pilT Acinetobacter baumannii D1279779

70.381

99.128

0.698
  pilT Acinetobacter baumannii strain A118

70.381

99.128

0.698
  pilT Acinetobacter nosocomialis M2

70.088

99.128

0.695
  pilT Acinetobacter baylyi ADP1

69.501

99.128

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.965

0.642
  pilT Vibrio cholerae strain A1552

65.579

97.965

0.642
  pilT Legionella pneumophila strain Lp02

64.497

98.256

0.634
  pilT Legionella pneumophila strain ERS1305867

64.497

98.256

0.634
  pilT Neisseria meningitidis 8013

62.647

98.837

0.619
  pilT Neisseria gonorrhoeae MS11

62.353

98.837

0.616
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.17

99.419

0.509
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392