NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	ORU25_RS06520	Genome accession	NZ_CP111145
Coordinates	1301806..1302240 (+)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain PF9
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1296806..1307240

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ORU25_RS06490 (ORU25_06490)	tadA	1296917..1297417 (-)	501	WP_005755875.1	tRNA adenosine(34) deaminase TadA	-
ORU25_RS06495 (ORU25_06495)	-	1297432..1298283 (-)	852	WP_010906470.1	thymidylate synthase	-
ORU25_RS06500 (ORU25_06500)	lgt	1298280..1299092 (-)	813	WP_010906471.1	prolipoprotein diacylglyceryl transferase	-
ORU25_RS06505 (ORU25_06505)	-	1299102..1299896 (-)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
ORU25_RS06510 (ORU25_06510)	rppH	1299899..1300495 (-)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
ORU25_RS06515 (ORU25_06515)	ampD	1301080..1301646 (-)	567	WP_005751074.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ORU25_RS06520 (ORU25_06520)	pilA	1301806..1302240 (+)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
ORU25_RS06525 (ORU25_06525)	pilB	1302240..1303625 (+)	1386	WP_010906472.1	GspE/PulE family protein	Machinery gene
ORU25_RS06530 (ORU25_06530)	-	1303625..1304836 (+)	1212	Protein_1267	type II secretion system F family protein	-
ORU25_RS06535 (ORU25_06535)	-	1304836..1305558 (+)	723	WP_010906474.1	prepilin peptidase	-
ORU25_RS06540 (ORU25_06540)	coaE	1305573..1306193 (+)	621	WP_010906475.1	dephospho-CoA kinase	-
ORU25_RS06545 (ORU25_06545)	yacG	1306204..1306407 (+)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
ORU25_RS06550 (ORU25_06550)	-	1306407..1306676 (+)	270	WP_005722568.1	GNAT family N-acetyltransferase	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=758162 ORU25_RS06520 WP_005741096.1 1301806..1302240(+) (pilA) [Pasteurella multocida strain PF9]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=758162 ORU25_RS06520 WP_005741096.1 1301806..1302240(+) (pilA) [Pasteurella multocida strain PF9]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC

CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACAGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0758162