Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OQ482_RS18630 Genome accession   NZ_CP110983
Coordinates   3896165..3897145 (-) Length   326 a.a.
NCBI ID   WP_248035506.1    Uniprot ID   -
Organism   Enterobacter bugandensis strain E104107     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3891165..3902145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQ482_RS18600 (OQ482_18595) - 3892055..3892552 (+) 498 WP_309264400.1 SprT family zinc-dependent metalloprotease -
  OQ482_RS18605 (OQ482_18600) endA 3892647..3893354 (+) 708 WP_309264401.1 deoxyribonuclease I -
  OQ482_RS18610 (OQ482_18605) rsmE 3893406..3894137 (+) 732 WP_041908327.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OQ482_RS18615 (OQ482_18610) gshB 3894157..3895104 (+) 948 WP_236300050.1 glutathione synthase -
  OQ482_RS18620 (OQ482_18615) - 3895179..3895739 (+) 561 WP_028014368.1 YqgE/AlgH family protein -
  OQ482_RS18625 (OQ482_18620) ruvX 3895739..3896155 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  OQ482_RS18630 (OQ482_18625) pilT 3896165..3897145 (-) 981 WP_248035506.1 type IV pilus twitching motility protein PilT Machinery gene
  OQ482_RS18635 (OQ482_18630) - 3897163..3897864 (+) 702 WP_047367421.1 YggS family pyridoxal phosphate-dependent enzyme -
  OQ482_RS18640 (OQ482_18635) - 3897886..3898452 (+) 567 WP_045260768.1 YggT family protein -
  OQ482_RS18645 (OQ482_18640) yggU 3898449..3898745 (+) 297 WP_047364545.1 DUF167 family protein YggU -
  OQ482_RS18650 (OQ482_18645) - 3898749..3899342 (+) 594 WP_010435473.1 XTP/dITP diphosphatase -
  OQ482_RS18655 (OQ482_18650) hemW 3899335..3900477 (+) 1143 WP_309264402.1 radical SAM family heme chaperone HemW -
  OQ482_RS18660 (OQ482_18655) - 3900541..3900879 (+) 339 WP_028014373.1 endonuclease domain-containing protein -
  OQ482_RS18665 (OQ482_18660) - 3900918..3902093 (-) 1176 WP_232929599.1 IS4 family transposase -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35528.57 Da        Isoelectric Point: 6.1627

>NTDB_id=757276 OQ482_RS18630 WP_248035506.1 3896165..3897145(-) (pilT) [Enterobacter bugandensis strain E104107]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNSHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=757276 OQ482_RS18630 WP_248035506.1 3896165..3897145(-) (pilT) [Enterobacter bugandensis strain E104107]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGATGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCTGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCGCTTGG
GGTGCCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAACGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGGTCGACTTCCTCAACAGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGTTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGTTGATCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCTGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Legionella pneumophila strain Lp02

50.311

98.773

0.497
  pilT Legionella pneumophila strain ERS1305867

50.311

98.773

0.497
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Acinetobacter baylyi ADP1

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

48.624

100

0.488
  pilT Acinetobacter baumannii strain A118

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

49.062

98.16

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362