Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KI231_RS27610 Genome accession   NZ_AP021903
Coordinates   6138121..6139155 (-) Length   344 a.a.
NCBI ID   WP_213026844.1    Uniprot ID   -
Organism   Pseudomonas sp. Seg1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6133121..6144155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI231_RS27580 (PHLH6_54400) ruvX 6133269..6133706 (+) 438 WP_064589634.1 Holliday junction resolvase RuvX -
  KI231_RS27585 (PHLH6_54410) pyrR 6133788..6134294 (+) 507 WP_103304300.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  KI231_RS27590 (PHLH6_54420) - 6134322..6135326 (+) 1005 WP_095164512.1 aspartate carbamoyltransferase catalytic subunit -
  KI231_RS27595 (PHLH6_54430) - 6135323..6136594 (+) 1272 WP_213026843.1 dihydroorotase -
  KI231_RS27600 (PHLH6_54440) - 6136695..6137129 (-) 435 WP_103304302.1 TM2 domain-containing protein -
  KI231_RS27605 (PHLH6_54450) - 6137378..6138001 (+) 624 WP_201236439.1 C40 family peptidase -
  KI231_RS27610 (PHLH6_54460) pilT 6138121..6139155 (-) 1035 WP_213026844.1 type IV pilus twitching motility protein PilT Machinery gene
  KI231_RS27615 (PHLH6_54470) - 6139212..6139898 (+) 687 WP_103304305.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI231_RS27620 (PHLH6_54480) proC 6139923..6140741 (+) 819 WP_103304306.1 pyrroline-5-carboxylate reductase -
  KI231_RS27625 (PHLH6_54490) - 6140752..6141342 (+) 591 WP_213026845.1 YggT family protein -
  KI231_RS27630 (PHLH6_54500) - 6141339..6141629 (+) 291 WP_213026846.1 DUF167 domain-containing protein -
  KI231_RS27635 (PHLH6_54510) - 6141866..6143004 (+) 1139 Protein_5453 homoserine O-acetyltransferase -
  KI231_RS27640 (PHLH6_54520) metW 6143012..6143632 (+) 621 WP_103304310.1 methionine biosynthesis protein MetW -
  KI231_RS27645 (PHLH6_54530) - 6143658..6144092 (+) 435 WP_213026848.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38041.71 Da        Isoelectric Point: 7.0157

>NTDB_id=75697 KI231_RS27610 WP_213026844.1 6138121..6139155(-) (pilT) [Pseudomonas sp. Seg1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=75697 KI231_RS27610 WP_213026844.1 6138121..6139155(-) (pilT) [Pseudomonas sp. Seg1]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGAGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATTCGAGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGCGTCGACTTCGAGAAACACCTGGAAACGGATTTTTCCTTCGAGGTCCCCGGTGTTGCACGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTCCGCACCATTCCATCGAAGGTGCTGAGCATGGA
AGACCTCGGCATGGGCGACGTCTTTCGCAAGATCACCGATGCCCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGCT
CCGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACCTCAATACCCATCGCCATCACCACATCCTCACCATCGAAGAC
CCGATCGAATTCGTTCACGAATCGCGCAAATGCCTGATCAACCAGCGCGAAGTCCACCGCGATACCCGCAGCTTCGCCAC
CGCGTTGCGTTCAGCACTGCGCGAAGATCCGGATGTGATTCTGGTGGGCGAGATGCGTGATCTGGAAACCATCCGCCTGG
CCCTGACCGCTGCCGAGACCGGGCATCTGGTGTTCGGCACGCTGCACACCACCTCGGCGGCGAAAACCATCGACCGGGTG
GTCGACGTATTCCCCGGTGATGAGAAGTCGATGGTGCGCTCGATGCTGTCGGAGTCGTTATTGGCAGTTGTGTCCCAGAC
GCTGATCAAGAAGATCGGCGGCGGACGGGTGGCGGCGCACGAGATCATGCTGGGAACGTCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACGGGTGGGGCGTTGGGGATGCAGACACTCGACATGTGCCTG
AAGGACCTGGTGACCAAGGGCTTGATCAGCCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment