Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ODU73_RS06250 Genome accession   NZ_CP110889
Coordinates   1361578..1362636 (+) Length   352 a.a.
NCBI ID   WP_265976728.1    Uniprot ID   -
Organism   Thermoclostridium stercorarium strain RKWS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1356578..1367636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ODU73_RS06230 (ODU73_001246) - 1356907..1357119 (+) 213 WP_015358935.1 DUF3006 domain-containing protein -
  ODU73_RS06240 (ODU73_001248) - 1357701..1359083 (-) 1383 WP_265976727.1 MATE family efflux transporter -
  ODU73_RS06245 (ODU73_001249) - 1359410..1361566 (+) 2157 WP_015358937.1 type II/IV secretion system protein -
  ODU73_RS06250 (ODU73_001250) pilT 1361578..1362636 (+) 1059 WP_265976728.1 type IV pilus twitching motility protein PilT Machinery gene
  ODU73_RS06255 (ODU73_001251) pilM 1362667..1363764 (+) 1098 WP_015358939.1 type IV pilus assembly protein PilM -
  ODU73_RS06260 (ODU73_001252) - 1363777..1364994 (+) 1218 WP_015358940.1 type II secretion system F family protein -
  ODU73_RS06265 (ODU73_001253) - 1365101..1365661 (+) 561 WP_265976729.1 type II secretion system protein -
  ODU73_RS06270 (ODU73_001254) - 1365790..1366209 (+) 420 WP_015358942.1 hypothetical protein -
  ODU73_RS06275 (ODU73_001255) - 1366481..1366861 (+) 381 WP_265976730.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38872.81 Da        Isoelectric Point: 6.6322

>NTDB_id=756853 ODU73_RS06250 WP_265976728.1 1361578..1362636(+) (pilT) [Thermoclostridium stercorarium strain RKWS1]
MNINEYLQKAVEMDASDLHICAGLPVVIRKHGSLFFIDDHILTGEDCLSLVKQLLNDSQFAFLEEEGEIDFAYMLSGVGR
FRVNAFRQRGTYAIAIRVIPSRVPKISELGLPDILYSLAERQRGLVLITGPTGSGKSTTLAAMIDHINSTKRCHIVTIED
PIEYLHKHNKSVINQREIGSDTKSYSNALRAVLREDPDVILIGEMRDLETISIALTAAETGHLVLSTLHTLGAAKTVDRI
IDVFPPHQQGQVRSQLSTVLQGIVSQQLIKKADNAGRVVATEVMIVTPAIANMIRESRTYQINSSIHTGGEYGMHSMDAC
IASLYKRGLITYENACQYAMDPVAMKKLIALE

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=756853 ODU73_RS06250 WP_265976728.1 1361578..1362636(+) (pilT) [Thermoclostridium stercorarium strain RKWS1]
ATGAATATCAACGAATACTTGCAAAAAGCCGTAGAGATGGATGCTTCAGACCTTCATATTTGTGCAGGTTTACCGGTGGT
CATAAGAAAACACGGCTCGCTGTTTTTTATCGATGACCACATACTTACAGGAGAAGATTGCCTGAGTTTGGTGAAGCAGT
TGTTGAACGACTCGCAGTTCGCGTTTCTTGAAGAAGAAGGAGAAATTGACTTTGCTTATATGCTCTCCGGTGTCGGAAGA
TTCAGGGTCAATGCTTTCAGGCAAAGAGGAACTTATGCCATAGCCATAAGGGTTATTCCGTCGAGAGTACCGAAAATCAG
TGAATTGGGGCTTCCGGATATCCTTTATTCACTGGCGGAAAGGCAGAGAGGCCTTGTCCTTATAACAGGGCCTACCGGAA
GCGGTAAATCCACAACCCTTGCCGCAATGATAGATCATATTAACAGTACAAAAAGATGCCACATTGTAACCATTGAAGAT
CCTATCGAATATTTGCATAAACACAACAAAAGCGTGATAAATCAGCGTGAAATAGGCTCGGATACTAAAAGTTACAGCAA
CGCATTGAGAGCAGTGTTAAGAGAAGATCCCGACGTTATTCTGATTGGTGAGATGAGAGATTTGGAGACAATTTCCATTG
CGTTAACCGCTGCTGAAACGGGACATCTGGTTCTGTCAACACTGCATACATTGGGAGCGGCCAAGACTGTTGACAGAATT
ATAGACGTATTCCCTCCGCATCAGCAAGGGCAGGTACGCAGCCAGCTGTCGACGGTGCTACAGGGGATTGTTTCGCAGCA
GTTGATAAAGAAAGCTGATAATGCGGGAAGAGTGGTCGCTACAGAGGTCATGATAGTAACTCCTGCGATAGCAAACATGA
TAAGGGAGTCGAGGACGTATCAGATCAACAGCAGCATTCATACGGGCGGAGAATACGGCATGCATTCCATGGATGCGTGC
ATAGCTTCTTTATATAAACGCGGTTTGATTACTTATGAAAATGCATGCCAGTATGCCATGGACCCTGTGGCAATGAAAAA
GTTAATAGCGCTGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.339

97.159

0.509
  pilT Neisseria meningitidis 8013

51.156

98.295

0.503
  pilT Neisseria gonorrhoeae MS11

50.867

98.295

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.864

0.494
  pilT Pseudomonas stutzeri DSM 10701

50.877

97.159

0.494
  pilT Acinetobacter nosocomialis M2

50.292

97.159

0.489
  pilT Acinetobacter baumannii D1279779

50.292

97.159

0.489
  pilT Acinetobacter baumannii strain A118

50.292

97.159

0.489
  pilT Acinetobacter baylyi ADP1

49.708

97.159

0.483
  pilT Legionella pneumophila strain Lp02

49.56

96.875

0.48
  pilT Legionella pneumophila strain ERS1305867

49.56

96.875

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.057

94.034

0.48
  pilT Vibrio cholerae strain A1552

51.057

94.034

0.48
  pilU Pseudomonas stutzeri DSM 10701

41.311

99.716

0.412
  pilU Acinetobacter baylyi ADP1

39.489

100

0.395
  pilU Vibrio cholerae strain A1552

39.528

96.307

0.381