Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OEI98_RS06330 Genome accession   NZ_CP110888
Coordinates   1239881..1240930 (+) Length   349 a.a.
NCBI ID   WP_266040291.1    Uniprot ID   -
Organism   Thermoanaerobacter sp. RKWS2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1234881..1245930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEI98_RS06310 (OEI98_001262) sigK 1235987..1236681 (+) 695 Protein_1198 RNA polymerase sporulation sigma factor SigK -
  OEI98_RS06315 (OEI98_001263) - 1236745..1237245 (+) 501 WP_266040287.1 YqeG family HAD IIIA-type phosphatase -
  OEI98_RS06320 (OEI98_001264) aroE 1237235..1238089 (+) 855 WP_266040289.1 shikimate dehydrogenase -
  OEI98_RS06325 (OEI98_001265) gspE 1238202..1239875 (+) 1674 Protein_1201 type II secretion system ATPase GspE -
  OEI98_RS06330 (OEI98_001266) pilT 1239881..1240930 (+) 1050 WP_266040291.1 type IV pilus twitching motility protein PilT Machinery gene
  OEI98_RS06335 (OEI98_001267) - 1240941..1242152 (+) 1212 WP_266040293.1 type II secretion system F family protein -
  OEI98_RS06340 (OEI98_001268) - 1242177..1242566 (+) 390 WP_266040295.1 type II secretion system protein -
  OEI98_RS06345 (OEI98_001269) - 1242616..1243374 (+) 759 WP_266040297.1 prepilin peptidase -
  OEI98_RS06350 (OEI98_001270) - 1243379..1243828 (+) 450 WP_266040299.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OEI98_RS06355 (OEI98_001271) - 1243839..1244516 (+) 678 WP_266040301.1 type IV pilus modification PilV family protein -
  OEI98_RS06360 (OEI98_001272) - 1244507..1244971 (+) 465 WP_266040303.1 PilW family protein -
  OEI98_RS06365 (OEI98_001273) - 1244961..1245494 (+) 534 WP_266040305.1 pilus assembly PilX family protein -
  OEI98_RS06370 (OEI98_001274) - 1245510..1245770 (+) 261 WP_003869819.1 late competence development ComFB family protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38613.88 Da        Isoelectric Point: 7.2221

>NTDB_id=756836 OEI98_RS06330 WP_266040291.1 1239881..1240930(+) (pilT) [Thermoanaerobacter sp. RKWS2]
MDTKELLKLVVERKASDLHITVGVPPVLRINGYLEKLEGEPFTPGQTEEIVKDLLTSEQLKKLEQNGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPPILKDLALKARGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDAASYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIKIQLSNVLEGIISQQLIPKKDNSGRVVATEVMIATPAIRNLIREGKTFQIQSAVQTGSKFGMMTMDMS
ILHLLKKGVISLEDALTYCVDQENFSRMI

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=756836 OEI98_RS06330 WP_266040291.1 1239881..1240930(+) (pilT) [Thermoanaerobacter sp. RKWS2]
ATGGATACAAAGGAACTTTTAAAATTAGTAGTTGAAAGAAAAGCTTCAGACTTGCATATAACGGTTGGAGTACCTCCAGT
TCTTAGAATTAATGGCTATTTAGAAAAATTAGAAGGGGAGCCTTTTACCCCTGGGCAGACTGAAGAAATAGTTAAGGATT
TATTGACAAGTGAGCAATTGAAAAAGTTAGAACAAAATGGAGATATTGATTTGTCCTATTCAGTTACAGGATTAGGAAGG
TTTAGAATAAATGTATACAAACAAAGGGGAAGTTATAGTCTTGCAATAAGGTCTGTAGCTTTGAGAATACCTACAATAGA
GGAATTGGGACTTCCTCCAATACTTAAAGACTTAGCTTTAAAGGCGAGAGGGCTTATATTGGTAACAGGACCTACTGGCA
GTGGTAAATCTACTACACTGGCTGCGATGGTGGATTGGATAAACTCTAAAAGGACTTGTCATATTCTCACATTGGAAGAC
CCTATAGAATATTTACATAAACACAACAAAAGCATAGTAAATCAAAGAGAGATTGGGCATGATGCGGCATCTTACGCTTC
AGCTTTAAGGGCTGCTTTAAGGGAGGACCCGGATGTTATATTAGTGGGAGAGATGAGGGATTTAGAAACCATCCAAATTG
CTATAACTGCTGCTGAGACAGGCCATCTTGTTTTGTCCACTCTTCACACAATAGGAGCCGTTAAGACGATCGATAGGATT
ATTGATGTTTTTCCTCCCCATCAGCAGCAGCAGATAAAAATTCAGCTTTCAAATGTTTTAGAGGGAATTATTTCTCAACA
GCTTATACCTAAAAAGGACAACAGCGGCAGAGTAGTTGCGACAGAAGTGATGATTGCTACTCCTGCTATAAGAAATCTCA
TAAGAGAAGGGAAGACTTTCCAGATACAGTCGGCAGTTCAAACAGGTAGCAAGTTTGGAATGATGACAATGGATATGTCT
ATTTTACATCTGTTAAAAAAAGGAGTTATTTCTTTGGAGGATGCATTGACCTATTGTGTGGACCAAGAGAATTTTTCTCG
CATGATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

49.855

98.854

0.493
  pilT Pseudomonas aeruginosa PAK

49.565

98.854

0.49
  pilT Legionella pneumophila strain ERS1305867

49.275

98.854

0.487
  pilT Legionella pneumophila strain Lp02

49.275

98.854

0.487
  pilT Acinetobacter nosocomialis M2

48.986

98.854

0.484
  pilT Acinetobacter baumannii D1279779

48.986

98.854

0.484
  pilT Acinetobacter baumannii strain A118

48.986

98.854

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.414

99.713

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.826

98.854

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.102

95.702

0.47
  pilT Vibrio cholerae strain A1552

49.102

95.702

0.47
  pilT Neisseria meningitidis 8013

45.954

99.14

0.456
  pilT Neisseria gonorrhoeae MS11

45.665

99.14

0.453
  pilU Pseudomonas stutzeri DSM 10701

41.834

100

0.418
  pilU Vibrio cholerae strain A1552

40.401

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

99.14

0.39