Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KI241_RS25595 Genome accession   NZ_AP021902
Coordinates   5818609..5819643 (-) Length   344 a.a.
NCBI ID   WP_053147214.1    Uniprot ID   -
Organism   Pseudomonas sp. Cab53     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5813609..5824643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI241_RS25565 (PHLH5_51140) ruvX 5813709..5814146 (+) 438 WP_042731346.1 Holliday junction resolvase RuvX -
  KI241_RS25570 (PHLH5_51150) pyrR 5814231..5814737 (+) 507 WP_047735856.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  KI241_RS25575 (PHLH5_51160) - 5814762..5815766 (+) 1005 WP_212803126.1 aspartate carbamoyltransferase catalytic subunit -
  KI241_RS25580 (PHLH5_51170) - 5815763..5817034 (+) 1272 WP_212803127.1 dihydroorotase -
  KI241_RS25585 (PHLH5_51180) - 5817158..5817586 (-) 429 WP_042731350.1 TM2 domain-containing protein -
  KI241_RS25590 (PHLH5_51190) - 5817835..5818446 (+) 612 WP_042731351.1 C40 family peptidase -
  KI241_RS25595 (PHLH5_51200) pilT 5818609..5819643 (-) 1035 WP_053147214.1 type IV pilus twitching motility protein PilT Machinery gene
  KI241_RS25600 (PHLH5_51210) - 5819699..5820385 (+) 687 WP_212803128.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI241_RS25605 (PHLH5_51220) proC 5820428..5821246 (+) 819 WP_212803129.1 pyrroline-5-carboxylate reductase -
  KI241_RS25610 (PHLH5_51230) - 5821256..5821846 (+) 591 WP_042732791.1 YggT family protein -
  KI241_RS25615 (PHLH5_51240) - 5822046..5823185 (+) 1140 WP_042731260.1 homoserine O-acetyltransferase -
  KI241_RS25620 (PHLH5_51250) metW 5823193..5823813 (+) 621 WP_042731259.1 methionine biosynthesis protein MetW -
  KI241_RS25625 (PHLH5_51260) - 5823839..5824273 (+) 435 WP_042731258.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38280.89 Da        Isoelectric Point: 6.8286

>NTDB_id=75668 KI241_RS25595 WP_053147214.1 5818609..5819643(-) (pilT) [Pseudomonas sp. Cab53]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTESPRGLVLVTGPTGSGKSTTLAAMIDYLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQDFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLIKKIGGGRIAAHEIMLGTSAIRNLIREDKIAQMYSSIQTGGSLGMQTLDMCL
KDLVNKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=75668 KI241_RS25595 WP_053147214.1 5818609..5819643(-) (pilT) [Pseudomonas sp. Cab53]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGGGCGTCGGACCTGCACCTGTCCGCCGGCTTGCCGCCGAT
GATCCGGGTCGATGGCGATGTGCGGCGGATCAACCTGCCGGCACTGGATCACAAGCAGGTCCATGAGTTGATCTACGACA
TCATGAACGACCGGCAACGAGTGGATTACGAAAAGTTTCTGGAAACCGACTTTTCCTTCGAGGTGCCGGGCGTTGCACGG
TTCCGGGTCAACGCCTTCAACCAGAATCGCGGCGCGGGGGCGGTGTTCCGGACCATCCCGTCGAAGGTCCTGACCATGGA
AGACCTGGGGATGGGCGAAGTGTTTCGCAAAGTGACCGAGTCGCCCCGGGGCCTGGTCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCGATGATCGACTACCTCAACAGCCACAAGCACCATCACATTCTTACCATCGAAGAC
CCGATCGAGTTCGTCCACGAGCCGCGCAAGTGCCTGATCAACCAGCGTGAGGTACACCGCGATACCCAGGACTTCTCCAC
GGCCTTGCGCTCGGCCCTGCGGGAAGACCCGGACGTGATCTTGGTGGGGGAAATGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCCGAAACCGGGCACCTGGTGTTCGGTACGCTGCACACCACGTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTATTCCCGGGGGACGAGAAGTCCATGGTGCGCTCGATGCTGTCGGAGTCACTGCAAGCGGTGATCTCCCAGAC
CCTGATCAAGAAGATCGGTGGCGGCCGCATTGCCGCGCACGAGATCATGCTGGGCACGTCGGCCATTCGCAACCTGATCC
GCGAAGACAAGATCGCCCAGATGTACTCGTCGATCCAGACCGGCGGGTCGCTGGGGATGCAGACGCTGGACATGTGCCTG
AAGGACCTGGTGAACAAGGGCCTGATCAGCCGGGAACATGCGCGGGAGCGGGCGCGTACGCCGGATAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Pseudomonas aeruginosa PAK

88.372

100

0.884
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

73.373

98.256

0.721
  pilT Legionella pneumophila strain ERS1305867

73.373

98.256

0.721
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392

Multiple sequence alignment