Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OQ460_RS24040 Genome accession   NZ_CP110853
Coordinates   5249892..5250926 (-) Length   344 a.a.
NCBI ID   WP_410019610.1    Uniprot ID   -
Organism   Pseudomonas sp. 16FHM2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5244892..5255926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQ460_RS24010 (OQ460_23940) ruvX 5245126..5245563 (+) 438 WP_410019604.1 Holliday junction resolvase RuvX -
  OQ460_RS24015 (OQ460_23945) pyrR 5245645..5246151 (+) 507 WP_410019605.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  OQ460_RS24020 (OQ460_23950) - 5246176..5247180 (+) 1005 WP_410019606.1 aspartate carbamoyltransferase catalytic subunit -
  OQ460_RS24025 (OQ460_23955) - 5247177..5248448 (+) 1272 WP_410019607.1 dihydroorotase -
  OQ460_RS24030 (OQ460_23960) - 5248527..5248955 (-) 429 WP_410019608.1 NINE protein -
  OQ460_RS24035 (OQ460_23965) - 5249205..5249840 (+) 636 WP_410019609.1 C40 family peptidase -
  OQ460_RS24040 (OQ460_23970) pilT 5249892..5250926 (-) 1035 WP_410019610.1 type IV pilus twitching motility protein PilT Machinery gene
  OQ460_RS24045 (OQ460_23975) - 5250984..5251670 (+) 687 WP_410019611.1 YggS family pyridoxal phosphate-dependent enzyme -
  OQ460_RS24050 (OQ460_23980) proC 5251704..5252522 (+) 819 WP_410019612.1 pyrroline-5-carboxylate reductase -
  OQ460_RS24055 (OQ460_23985) - 5252532..5253122 (+) 591 WP_410019613.1 YggT family protein -
  OQ460_RS24060 (OQ460_23990) - 5253330..5254469 (+) 1140 WP_410019614.1 homoserine O-acetyltransferase -
  OQ460_RS24065 (OQ460_23995) metW 5254477..5255097 (+) 621 WP_003213768.1 methionine biosynthesis protein MetW -
  OQ460_RS24070 (OQ460_24000) - 5255119..5255556 (+) 438 WP_410019615.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37654.31 Da        Isoelectric Point: 6.7288

>NTDB_id=756665 OQ460_RS24040 WP_410019610.1 5249892..5250926(-) (pilT) [Pseudomonas sp. 16FHM2]
MDITELLAESVSLGASDLHLSAGLAPRVRVDGDIHPLDWPVLSATQVADMLSPLLNAHQQKDFETSLETDFSFELPEVAR
FRVNVFRQHRGMGAVFRAIPSRVLSLEELQLGDVFRRIADLPQGLVLVTGPTGSGKSTTLAAMIDYLNNHRPRHILTLED
PIEFVHPSQKALVSQRQVYRDTHSFCAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFATLHTRSAAKTVDRV
VDVFPANEKAIVRSMLSESLQAVVSQALVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMYSVIQTGGAFGMKTLDMSL
KALVSQGLISREEARQSARAPGEM

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=756665 OQ460_RS24040 WP_410019610.1 5249892..5250926(-) (pilT) [Pseudomonas sp. 16FHM2]
ATGGATATCACTGAACTGCTGGCCGAAAGCGTCAGCCTCGGCGCTTCGGACCTGCATTTGTCGGCCGGTCTGGCGCCGAG
GGTGCGGGTCGATGGCGACATTCACCCCCTCGACTGGCCCGTGCTGAGCGCTACGCAAGTGGCGGATATGCTCAGTCCCT
TGCTCAATGCACACCAGCAAAAGGATTTCGAAACATCTCTTGAAACGGACTTTTCGTTCGAGTTGCCTGAGGTGGCGCGT
TTTCGCGTGAATGTGTTCCGGCAGCACCGAGGAATGGGCGCGGTATTTCGCGCGATTCCGTCACGGGTACTCAGTCTCGA
GGAACTGCAACTGGGAGATGTGTTCCGGCGTATTGCCGATTTGCCCCAGGGGCTGGTGCTGGTGACCGGGCCTACAGGCT
CGGGAAAATCCACCACCCTGGCGGCAATGATCGATTACCTCAACAACCATCGCCCACGACATATCCTCACACTTGAAGAT
CCCATCGAGTTTGTTCATCCCTCACAAAAAGCGCTGGTCAGTCAGCGCCAGGTGTACCGCGACACCCACAGCTTCTGTGC
AGCCCTGCGCTCTGCTCTGCGCGAGGACCCGGATGTGATTCTGGTGGGTGAAATGCGTGATCTGGAAACCATTCGCCTGG
CCCTGACGGCGGCGGAAACCGGGCATCTGGTATTCGCGACCCTGCATACCCGATCGGCGGCAAAAACCGTGGATCGGGTG
GTGGATGTGTTTCCCGCCAATGAAAAGGCCATCGTTCGCTCAATGCTTTCGGAGTCGTTGCAGGCGGTGGTGTCTCAAGC
GCTGGTCAAGAAGGTCGGCGGCGGGCGGGTGGCGGCGCATGAAATCATGCTTGGTACACCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTAGCGCAGATGTACTCGGTGATTCAGACCGGTGGGGCGTTCGGGATGAAGACGTTGGACATGAGCTTG
AAGGCGTTGGTCAGCCAAGGGCTGATCAGCCGTGAGGAGGCCCGGCAGAGCGCGCGGGCGCCCGGGGAAATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.9

99.128

0.733
  pilT Pseudomonas stutzeri DSM 10701

72.434

99.128

0.718
  pilT Acinetobacter nosocomialis M2

69.795

99.128

0.692
  pilT Acinetobacter baumannii D1279779

69.501

99.128

0.689
  pilT Acinetobacter baumannii strain A118

69.501

99.128

0.689
  pilT Acinetobacter baylyi ADP1

69.208

99.128

0.686
  pilT Neisseria meningitidis 8013

63.478

100

0.637
  pilT Legionella pneumophila strain Lp02

64.497

98.256

0.634
  pilT Legionella pneumophila strain ERS1305867

64.497

98.256

0.634
  pilT Neisseria gonorrhoeae MS11

63.188

100

0.634
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.284

97.384

0.616
  pilT Vibrio cholerae strain A1552

63.284

97.384

0.616
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Vibrio cholerae strain A1552

38.841

100

0.39
  pilU Acinetobacter baylyi ADP1

36.416

100

0.366