Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OKW98_RS03110 Genome accession   NZ_CP110644
Coordinates   674686..675720 (+) Length   344 a.a.
NCBI ID   WP_265387931.1    Uniprot ID   -
Organism   Pseudomonas sp. KU26590     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 669686..680720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OKW98_RS03080 (OKW98_03080) rdgB 669686..670294 (-) 609 WP_265389637.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OKW98_RS03085 (OKW98_03085) metW 670419..671039 (-) 621 WP_265387927.1 methionine biosynthesis protein MetW -
  OKW98_RS03090 (OKW98_03090) metX 671047..672186 (-) 1140 WP_265387928.1 homoserine O-succinyltransferase MetX -
  OKW98_RS03095 (OKW98_03095) - 672501..673091 (-) 591 WP_065991147.1 YggT family protein -
  OKW98_RS03100 (OKW98_03100) proC 673102..673920 (-) 819 WP_265387929.1 pyrroline-5-carboxylate reductase -
  OKW98_RS03105 (OKW98_03105) - 673945..674631 (-) 687 WP_265387930.1 YggS family pyridoxal phosphate-dependent enzyme -
  OKW98_RS03110 (OKW98_03110) pilT 674686..675720 (+) 1035 WP_265387931.1 type IV pilus twitching motility protein PilT Machinery gene
  OKW98_RS03115 (OKW98_03115) - 675839..676570 (-) 732 WP_265389638.1 C40 family peptidase -
  OKW98_RS03120 (OKW98_03120) - 676821..677237 (+) 417 WP_265387932.1 NINE protein -
  OKW98_RS03125 (OKW98_03125) - 677575..678846 (-) 1272 WP_265387933.1 dihydroorotase -
  OKW98_RS03130 (OKW98_03130) - 678843..679847 (-) 1005 WP_192135527.1 aspartate carbamoyltransferase catalytic subunit -
  OKW98_RS03135 (OKW98_03135) pyrR 679860..680381 (-) 522 WP_265387934.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37964.54 Da        Isoelectric Point: 6.7841

>NTDB_id=755586 OKW98_RS03110 WP_265387931.1 674686..675720(+) (pilT) [Pseudomonas sp. KU26590]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDQKEVQALIYDIMNDRQRKDFEEHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMVDYLNSNKHHHILTIED
PIEFVHESKKSLINQREVHRDTHGFSEALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKSIDRI
VDVFPAQEKSMIRSMLSESLHAIVSQALLKKVGGGRVAAHEIMIGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLISKGLITRESAREKAKTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=755586 OKW98_RS03110 WP_265387931.1 674686..675720(+) (pilT) [Pseudomonas sp. KU26590]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAGCAAGGCGCGTCGGACTTGCATCTCTCTGCCGGCCTGCCACCGAT
GATTCGCGTCGATGGCGATGTGCGGCGGATCAACCTGCCCGCACTGGACCAGAAAGAGGTTCAGGCGCTGATCTACGACA
TCATGAACGACAGGCAGCGCAAGGACTTCGAGGAACATCTGGAGACGGATTTTTCCTTCGAGGTGCCGGGCGTCGCGCGC
TTCCGGGTCAACGCGTTCAACCAGAATCGCGGCGCGGGCGCGGTGTTCCGGACCATTCCGTCGAAGGTCCTGAGCATGGA
AGAGCTGGGCATGGGCGAAGTGTTTCGCAAGATTACCGACGTGCCCCGCGGCCTGGTTCTGGTCACCGGTCCGACCGGTT
CGGGCAAATCGACCACTCTGGCAGCCATGGTCGATTACCTGAACAGCAACAAGCATCACCACATCCTCACCATCGAAGAC
CCGATCGAATTCGTCCATGAGTCCAAGAAAAGCCTGATCAACCAGCGCGAAGTTCATCGCGATACTCACGGCTTCTCCGA
GGCACTGCGTTCAGCGTTGCGTGAAGACCCTGACGTGATTCTGGTGGGTGAGCTGCGTGACCTGGAAACCATTCGCCTGG
CGCTGACGGCAGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCACGTCTGCTGCCAAGAGCATCGACCGGATC
GTCGACGTGTTTCCGGCGCAGGAGAAGTCGATGATCCGCTCGATGCTCTCCGAGTCGCTCCACGCCATCGTGTCTCAGGC
GCTGCTGAAGAAGGTCGGCGGGGGGCGTGTCGCGGCCCACGAGATCATGATCGGCACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCGATTCAGACCGGGGGATCACTGGGGATGCAGACGCTGGATATGTGCCTG
AAGGATCTGATCAGCAAGGGGCTGATCACGCGCGAGAGTGCGCGGGAGAAGGCGAAGACACCGGATAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.535

100

0.895
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Vibrio cholerae strain A1552

38.841

100

0.39