Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OM794_RS02055 Genome accession   NZ_CP110535
Coordinates   396770..397822 (+) Length   350 a.a.
NCBI ID   WP_226250360.1    Uniprot ID   -
Organism   Halomonas sp. BDJS001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 391770..402822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM794_RS02025 (OM794_02025) - 392093..392542 (-) 450 WP_226250356.1 DUF4426 domain-containing protein -
  OM794_RS02030 (OM794_02030) metW 392584..393177 (-) 594 WP_226250368.1 methionine biosynthesis protein MetW -
  OM794_RS02035 (OM794_02035) metX 393177..394403 (-) 1227 WP_226250357.1 homoserine O-succinyltransferase MetX -
  OM794_RS02040 (OM794_02040) - 394490..395083 (-) 594 WP_088702216.1 YggT family protein -
  OM794_RS02045 (OM794_02045) proC 395141..395965 (-) 825 WP_226250358.1 pyrroline-5-carboxylate reductase -
  OM794_RS02050 (OM794_02050) - 396021..396737 (-) 717 WP_226250359.1 YggS family pyridoxal phosphate-dependent enzyme -
  OM794_RS02055 (OM794_02055) pilT 396770..397822 (+) 1053 WP_226250360.1 type IV pilus twitching motility protein PilT Machinery gene
  OM794_RS02060 (OM794_02060) - 397867..399006 (-) 1140 WP_226250361.1 anhydro-N-acetylmuramic acid kinase -
  OM794_RS02065 (OM794_02065) tyrS 399152..400351 (+) 1200 WP_226250362.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38500.17 Da        Isoelectric Point: 6.3487

>NTDB_id=755257 OM794_RS02055 WP_226250360.1 396770..397822(+) (pilT) [Halomonas sp. BDJS001]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNEVRKLIYDIMNDRQRRDYGEHLEIDFSFEIVGVAR
FRVNAFTQARGAGAVFRTIPNQVLSMQALGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHASKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVVSQSLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQMYSAIQTGGSLGMQTLDAAL
TRLVKEGVVSLQEAQMSAKGTLALKDDIER

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=755257 OM794_RS02055 WP_226250360.1 396770..397822(+) (pilT) [Halomonas sp. BDJS001]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCCTCTGACTTGCACCTTTCGGCCGGTTTGCCACCCAT
GATACGTGTTGATGGCGACATTCGTCGGCTCAATGTGCCCGCTATCGATAATAATGAAGTGCGCAAATTGATCTACGACA
TCATGAATGATCGACAGCGGCGCGACTACGGAGAGCACCTGGAAATTGATTTTTCCTTTGAAATCGTTGGGGTAGCGCGC
TTTCGTGTTAATGCGTTCACCCAGGCCCGCGGGGCGGGGGCGGTATTTCGTACCATTCCCAATCAAGTGCTTTCCATGCA
AGCCCTGGGGCTCGGTGAGGTGTTCGAGCGTCTGGCGATGTTGCCGCGGGGCCTGGTGCTGGTGACTGGGCCAACGGGGT
CGGGTAAGAGCACGACGCTGGCCGCGATGATCGATTACATTAACGATCATCGTTACGAACATATACTCACCATTGAAGAC
CCCGTCGAGTTTGTCCACGCCAGCAAGCGCTGTTTGATTAACCAACGCGAAGTGCATCGCGACACCCATAGCTTTGCCGA
TGCGTTGCGTAGCGCGCTGCGTGAAGACCCGGATGTGATTCTGGTCGGAGAACTGCGCGATCTGGAAACCATTCGTTTAG
CGCTGACCGCGGCTGAAACCGGCCACCTGGTGCTGGGAACGCTGCATACCACCTCTGCCGCCAAGACAATCGATCGGATC
ATTGATGTTTTTCCCGGCGAAGAGAAAGCAATGGTGCGCTCGATGCTGTCGGAATCGCTCCAGGCAGTGGTGTCGCAATC
GTTGCTTAAACGTCAGGGCGGCGGGCGCGTAGCGGCCCACGAAATCCTCATTGCCACTTCTGCAGTGCGCAACTTGATCC
GCGAAGACAAAGTGGCGCAGATGTACTCTGCCATTCAAACCGGCGGCAGCCTGGGTATGCAAACCCTGGATGCCGCGCTC
ACTCGGCTAGTGAAAGAGGGCGTGGTGAGTTTGCAAGAAGCGCAAATGAGCGCGAAGGGCACCTTGGCATTGAAAGATGA
CATTGAGAGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.286

96.857

0.749
  pilT Pseudomonas stutzeri DSM 10701

76.991

96.857

0.746
  pilT Acinetobacter nosocomialis M2

75.516

96.857

0.731
  pilT Acinetobacter baumannii D1279779

75.516

96.857

0.731
  pilT Acinetobacter baumannii strain A118

75.516

96.857

0.731
  pilT Acinetobacter baylyi ADP1

74.631

96.857

0.723
  pilT Legionella pneumophila strain Lp02

72.189

96.571

0.697
  pilT Legionella pneumophila strain ERS1305867

72.189

96.571

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.149

95.714

0.671
  pilT Vibrio cholerae strain A1552

70.149

95.714

0.671
  pilT Neisseria meningitidis 8013

65.205

97.714

0.637
  pilT Neisseria gonorrhoeae MS11

64.912

97.714

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.731

95.714

0.514
  pilU Pseudomonas stutzeri DSM 10701

41.329

98.857

0.409
  pilU Vibrio cholerae strain A1552

41.003

96.857

0.397
  pilU Acinetobacter baylyi ADP1

40.774

96

0.391