Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OM420_RS18385 Genome accession   NZ_CP110534
Coordinates   3914849..3915829 (-) Length   326 a.a.
NCBI ID   WP_094944185.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain WS28-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3909849..3920829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM420_RS18355 (OM420_18345) - 3910730..3911227 (+) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -
  OM420_RS18360 (OM420_18350) endA 3911322..3912029 (+) 708 WP_021242074.1 deoxyribonuclease I -
  OM420_RS18365 (OM420_18355) rsmE 3912081..3912812 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OM420_RS18370 (OM420_18360) gshB 3912832..3913779 (+) 948 WP_008499751.1 glutathione synthase -
  OM420_RS18375 (OM420_18365) - 3913862..3914422 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  OM420_RS18380 (OM420_18370) ruvX 3914422..3914838 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  OM420_RS18385 (OM420_18375) pilT 3914849..3915829 (-) 981 WP_094944185.1 type IV pilus twitching motility protein PilT Machinery gene
  OM420_RS18390 (OM420_18380) - 3915847..3916548 (+) 702 WP_008499756.1 YggS family pyridoxal phosphate-dependent enzyme -
  OM420_RS18395 (OM420_18385) - 3916570..3917136 (+) 567 WP_094944187.1 YggT family protein -
  OM420_RS18400 (OM420_18390) yggU 3917133..3917429 (+) 297 WP_008499758.1 DUF167 family protein YggU -
  OM420_RS18405 (OM420_18395) - 3917433..3918026 (+) 594 WP_023294524.1 XTP/dITP diphosphatase -
  OM420_RS18410 (OM420_18400) hemW 3918019..3919167 (+) 1149 WP_063435943.1 radical SAM family heme chaperone HemW -
  OM420_RS18415 (OM420_18405) - 3919352..3920068 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  OM420_RS18420 (OM420_18410) - 3920125..3920451 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35407.43 Da        Isoelectric Point: 7.4841

>NTDB_id=755250 OM420_RS18385 WP_094944185.1 3914849..3915829(-) (pilT) [Enterobacter roggenkampii strain WS28-4]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPSPDLGALLKAWLNDEQQGIWWASGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRAIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTNGHILTLEDPVE
FIYQSARCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=755250 OM420_RS18385 WP_094944185.1 3914849..3915829(-) (pilT) [Enterobacter roggenkampii strain WS28-4]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCCTCTCCTGACCTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAGCGGGCAGGTGGATTTTGCCGCGACGGTGGCAGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTGACGCTACGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGCAATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTAACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGCACGTTGCCTGATCCAGCAGCGGGAGATCGGCCAGCACAGCCCGTCATTTGCCGATGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTACTGGGAGAGTTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
CGACCTGCAGGGTGGGCGCGTCGCGCTGTATGAACTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.41

98.773

0.439
  pilU Pseudomonas stutzeri DSM 10701

39.879

100

0.405
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368