Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OM418_RS18425 Genome accession   NZ_CP110533
Coordinates   3919437..3920417 (-) Length   326 a.a.
NCBI ID   WP_248165616.1    Uniprot ID   -
Organism   Enterobacter quasiroggenkampii strain WS12-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3914437..3925417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM418_RS18395 (OM418_18395) - 3915312..3915809 (+) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -
  OM418_RS18400 (OM418_18400) endA 3915904..3916611 (+) 708 WP_045909929.1 deoxyribonuclease I -
  OM418_RS18405 (OM418_18405) rsmE 3916664..3917395 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OM418_RS18410 (OM418_18410) gshB 3917415..3918362 (+) 948 WP_277718061.1 glutathione synthase -
  OM418_RS18415 (OM418_18415) - 3918450..3919010 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  OM418_RS18420 (OM418_18420) ruvX 3919010..3919426 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  OM418_RS18425 (OM418_18425) pilT 3919437..3920417 (-) 981 WP_248165616.1 type IV pilus twitching motility protein PilT Machinery gene
  OM418_RS18430 (OM418_18430) - 3920435..3921136 (+) 702 WP_045909932.1 YggS family pyridoxal phosphate-dependent enzyme -
  OM418_RS18435 (OM418_18435) - 3921158..3921724 (+) 567 WP_045909933.1 YggT family protein -
  OM418_RS18440 (OM418_18440) yggU 3921721..3922017 (+) 297 WP_045909934.1 DUF167 family protein YggU -
  OM418_RS18445 (OM418_18445) - 3922021..3922614 (+) 594 WP_025912468.1 XTP/dITP diphosphatase -
  OM418_RS18450 (OM418_18450) hemW 3922607..3923749 (+) 1143 WP_277718062.1 radical SAM family heme chaperone HemW -
  OM418_RS18455 (OM418_18455) - 3923813..3924175 (+) 363 WP_277718063.1 endonuclease domain-containing protein -
  OM418_RS18460 (OM418_18460) - 3924251..3924967 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  OM418_RS18465 (OM418_18465) - 3925024..3925350 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35522.56 Da        Isoelectric Point: 6.9108

>NTDB_id=755227 OM418_RS18425 WP_248165616.1 3919437..3920417(-) (pilT) [Enterobacter quasiroggenkampii strain WS12-3]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGIWWASGQVDFAATVSGGQRLRG
SAFKQMRGVSVTLRLLPRICPQLASLGAPRAIPELLTNESGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIRQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=755227 OM418_RS18425 WP_248165616.1 3919437..3920417(-) (pilT) [Enterobacter quasiroggenkampii strain WS12-3]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACAGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCCGACGTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAGCGGGCAGGTGGATTTTGCCGCGACGGTGTCGGGAGGTCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTATCTGTCCGCAGCTCGCTTCACTGGG
TGCGCCGAGGGCCATCCCGGAACTGTTAACCAATGAGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGTAAAT
CCACGACGCTGGCGGCGATGGTTGATTTTCTCAACCACCATACTGACGGCCATATCCTCACGCTCGAAGATCCGGTGGAA
TTTATCTACCAGAGCGAGCGTTGCCTGATCCGGCAGCGGGAGATTGGTCAGCACAGCCCGTCATTTGCCGACGCGCTGCG
CAGCGCCTTACGCCAGGATCCTGACGTGATTTTGCTCGGGGAGTTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CAGCAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACGCGCGGGGCATCGCAGGCGATCGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAACTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCGGCGGCGGCGAATCTGATACGTGAAGGAA
AAACGTGGCAACTTCCCGGCATCATTCAAACCGGTCAGCAGGCAGGCATGCAGAACTTTGACCAGAGTCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

98.773

0.436
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362