Detailed information NTG0755137

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: OM087_RS18605
• Genome accession: NZ_CP110511
• Coordinates: 3868009..3868989 (+)
• Length: 326 a.a.
• NCBI ID: WP_115724171.1
• Uniprot ID: -
• Organism: Escherichia coli strain ws5-3
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	3869216..3870196	3868009..3868989	flank	227

Gene organization within MGE regions

Location: 3868009..3870196

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OM087_RS18605 (OM087_18540)	pilT	3868009..3868989 (+)	981	WP_115724171.1	type IV pilus twitching motility protein PilT	Machinery gene
OM087_RS18610 (OM087_18545)	-	3869216..3870196 (+)	981	WP_000399648.1	IS110-like element IS621 family transposase	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35979.23 Da
• Isoelectric Point: 6.1257

>NTDB_id=755137 OM087_RS18605 WP_115724171.1 3868009..3868989(+) (pilT) [Escherichia coli strain ws5-3]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAEKQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=755137 OM087_RS18605 WP_115724171.1 3868009..3868989(+) (pilT) [Escherichia coli strain ws5-3]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAAACAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGTTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCTCCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (63-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	49.085	100	0.494
pilT	Neisseria gonorrhoeae MS11	48.78	100	0.491
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Acinetobacter nosocomialis M2	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain Lp02	46.541	97.546	0.454
pilT	Legionella pneumophila strain ERS1305867	46.541	97.546	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.453	97.546	0.414
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Acinetobacter baylyi ADP1	37.273	100	0.377
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377