Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LJ360_RS02205 Genome accession   NZ_CP110342
Coordinates   513422..514525 (-) Length   367 a.a.
NCBI ID   WP_264995113.1    Uniprot ID   A0A9E8B0D5
Organism   Marinobacter sp. AN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508422..519525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJ360_RS02180 (LJ360_02180) - 508551..509770 (-) 1220 Protein_434 ABC transporter substrate-binding protein -
  LJ360_RS02185 (LJ360_02185) - 509839..510558 (-) 720 WP_091848817.1 ABC transporter ATP-binding protein -
  LJ360_RS02190 (LJ360_02190) - 510548..511291 (-) 744 WP_413774412.1 ABC transporter ATP-binding protein -
  LJ360_RS02195 (LJ360_02195) - 511583..512344 (-) 762 WP_264995111.1 SDR family oxidoreductase -
  LJ360_RS02200 (LJ360_02200) - 512603..513334 (-) 732 WP_264995112.1 transporter substrate-binding domain-containing protein -
  LJ360_RS02205 (LJ360_02205) pilU 513422..514525 (-) 1104 WP_264995113.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LJ360_RS02210 (LJ360_02210) - 514540..516888 (-) 2349 WP_264995114.1 EAL domain-containing protein -
  LJ360_RS02215 (LJ360_02215) - 517124..517516 (-) 393 WP_264995115.1 globin -
  LJ360_RS02220 (LJ360_02220) - 517688..518131 (-) 444 WP_264995116.1 hypothetical protein -
  LJ360_RS02225 (LJ360_02225) - 518251..518589 (+) 339 WP_264995117.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41042.14 Da        Isoelectric Point: 6.8750

>NTDB_id=754123 LJ360_RS02205 WP_264995113.1 513422..514525(-) (pilU) [Marinobacter sp. AN1]
MELPAYLKVMADKGASDLYLSTGAPVMMKIEGKTGPVTQKPLPAGSVKELAYSVMSDRQRAEFEKNLELDMAVGISQVGR
FRLNVFWQRGEVAMVVRHLKTNIPSIESLGLPRVLEQLIMQPRGLILVVGATGSGKSTTLASMIDYRNTHRSGHILTIED
PIEYTHHHKQSVVNQREVGTDTLSYGEALRRAMREAPDVIMIGEIRDRETMKHALTYAETGHLCLATLHASNVNQTMRRI
VNFFPEHAHRELLMDLSMHLQAVVSQRLLKTLDGKRAAAVEVMLASPYIRELINKGELEGIGEIMEKSPEQGMQTFDHAI
LELYRQGRISREEALANADSRHNLEVRIRLEQGGLDSGNDQFSIDPS

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=754123 LJ360_RS02205 WP_264995113.1 513422..514525(-) (pilU) [Marinobacter sp. AN1]
ATGGAATTACCAGCCTACCTGAAAGTCATGGCCGACAAGGGCGCCTCGGACCTTTACCTGTCCACCGGTGCGCCGGTGAT
GATGAAGATCGAAGGCAAAACCGGCCCCGTCACCCAGAAGCCCCTGCCTGCCGGTTCCGTCAAGGAGCTGGCCTACTCGG
TTATGAGTGACCGCCAGCGGGCGGAATTCGAAAAGAACCTGGAGCTGGATATGGCTGTCGGTATCAGCCAGGTGGGCCGC
TTTCGGCTCAACGTCTTCTGGCAGCGGGGTGAGGTAGCGATGGTCGTGCGCCACCTGAAGACGAATATCCCGTCCATCGA
ATCCCTGGGGCTGCCCCGGGTGCTGGAGCAGCTGATCATGCAACCTCGTGGGCTGATACTGGTCGTGGGCGCCACGGGTT
CAGGCAAGTCCACGACCCTGGCATCCATGATCGACTACCGTAACACCCACCGCTCAGGGCACATCCTGACCATCGAGGAC
CCGATCGAGTACACCCATCACCATAAGCAAAGCGTGGTGAACCAGCGGGAAGTCGGTACCGACACCCTGTCCTACGGTGA
AGCCCTGCGCCGAGCCATGCGGGAAGCACCGGACGTGATCATGATCGGCGAGATCCGTGACCGGGAAACCATGAAGCACG
CCCTCACCTACGCCGAAACTGGCCACCTGTGCCTGGCTACACTGCACGCCAGTAACGTCAACCAGACCATGCGTCGCATC
GTCAATTTCTTCCCGGAGCACGCCCACCGGGAGCTGCTGATGGACCTGAGCATGCACCTGCAAGCCGTCGTATCGCAGCG
TCTGCTCAAGACCCTTGACGGCAAACGGGCCGCGGCAGTGGAAGTGATGCTTGCCAGCCCCTACATCCGGGAACTGATCA
ACAAGGGTGAACTGGAGGGCATCGGCGAAATCATGGAGAAGAGCCCGGAACAGGGCATGCAAACGTTCGACCACGCCATC
CTGGAACTCTACCGCCAGGGCCGGATTAGCCGCGAGGAAGCCCTCGCCAACGCGGACTCCCGCCATAATCTGGAGGTCAG
GATCCGCCTCGAACAGGGCGGCCTGGACTCCGGCAACGATCAGTTCAGTATTGACCCGTCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.427

95.64

0.482
  pilU Vibrio cholerae strain A1552

48.023

96.458

0.463
  pilU Acinetobacter baylyi ADP1

47.175

96.458

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.133

92.371

0.417
  pilT Pseudomonas aeruginosa PAK

43.605

93.733

0.409
  pilT Pseudomonas stutzeri DSM 10701

43.314

93.733

0.406
  pilT Neisseria meningitidis 8013

43.75

91.553

0.401
  pilT Acinetobacter baylyi ADP1

42.647

92.643

0.395
  pilT Legionella pneumophila strain Lp02

43.114

91.008

0.392
  pilT Legionella pneumophila strain ERS1305867

43.114

91.008

0.392
  pilT Neisseria gonorrhoeae MS11

42.985

91.281

0.392
  pilT Vibrio cholerae strain A1552

42.342

90.736

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.342

90.736

0.384
  pilT Acinetobacter nosocomialis M2

41.916

91.008

0.381
  pilT Acinetobacter baumannii D1279779

41.916

91.008

0.381
  pilT Acinetobacter baumannii strain A118

41.916

91.008

0.381