Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OM953_RS08135 Genome accession   NZ_CP110301
Coordinates   1846188..1847207 (+) Length   339 a.a.
NCBI ID   WP_264684745.1    Uniprot ID   -
Organism   Xanthomonas citri pv. fuscans strain CFBP 6533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1841188..1852207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM953_RS22335 - 1841437..1841979 (-) 543 WP_007966164.1 hypothetical protein -
  OM953_RS08110 (OM953_08110) - 1842036..1842377 (-) 342 WP_003489901.1 DUF3861 family protein -
  OM953_RS08115 (OM953_08115) - 1842435..1843076 (-) 642 WP_099802081.1 M23 family metallopeptidase -
  OM953_RS08120 (OM953_08120) - 1843179..1843604 (-) 426 WP_008571830.1 HU family DNA-binding protein -
  OM953_RS08125 (OM953_08125) proC 1844168..1845022 (-) 855 WP_099802080.1 pyrroline-5-carboxylate reductase -
  OM953_RS08130 (OM953_08130) - 1845066..1845758 (-) 693 WP_099802079.1 YggS family pyridoxal phosphate-dependent enzyme -
  OM953_RS08135 (OM953_08135) pilT 1846188..1847207 (+) 1020 WP_264684745.1 type IV pilus twitching motility protein PilT Machinery gene
  OM953_RS08140 (OM953_08140) pilU 1847320..1848450 (+) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OM953_RS08145 (OM953_08145) - 1848945..1849619 (+) 675 WP_162808471.1 YitT family protein -
  OM953_RS08150 (OM953_08150) - 1849794..1850720 (-) 927 WP_173644273.1 DUF72 domain-containing protein -
  OM953_RS08155 (OM953_08155) - 1850728..1851300 (-) 573 WP_078587306.1 DNA-3-methyladenine glycosylase I -
  OM953_RS08160 (OM953_08160) - 1851568..1852134 (+) 567 WP_003489930.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 37988.53 Da        Isoelectric Point: 7.0284

>NTDB_id=753981 OM953_RS08135 WP_264684745.1 1846188..1847207(+) (pilT) [Xanthomonas citri pv. fuscans strain CFBP 6533]
MAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLARFRVNAF
NQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVEDPIEFVH
TSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRIIDVFPA
GEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHLQDLVKR
SLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=753981 OM953_RS08135 WP_264684745.1 1846188..1847207(+) (pilT) [Xanthomonas citri pv. fuscans strain CFBP 6533]
TTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGATGATCCGTGTCGATGGCGA
TGTCCGTCGCATCAATATTCCGGCGTTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACATCATGTCGGACAAGCAGC
GTCGCGATTACGAAGAATTTCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGCTTCCGCGTCAATGCGTTC
AACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGAAGACCTTGGCTGCCCGCC
GATCTTCCGTCAGCTGATCGATCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGTCGACCACGC
TGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGGCACATCCTCACCGTCGAGGACCCGATCGAATTCGTCCAC
ACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGAAGCGCTGCGCTCGGCGCT
GCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATTCGCCTGGCGCTCACCGCTGCGGAAA
CCGGTCACTTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATCATCGACGTGTTCCCGGCC
GGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCTGTGATTTCGCAGGCGCTGTTGAAGAAGGTCGG
CGGCGGCCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCCGCGAGGACAAGGTGGCGC
AGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTCGACCAGCATCTGCAGGACCTGGTCAAGCGC
AGCCTGATCACGCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.586

99.705

0.794
  pilT Acinetobacter baumannii D1279779

79.586

99.705

0.794
  pilT Acinetobacter baumannii strain A118

79.586

99.705

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.994

99.705

0.788
  pilT Acinetobacter baylyi ADP1

78.402

99.705

0.782
  pilT Pseudomonas aeruginosa PAK

78.679

98.23

0.773
  pilT Legionella pneumophila strain Lp02

74.556

99.705

0.743
  pilT Legionella pneumophila strain ERS1305867

74.556

99.705

0.743
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.279

95.28

0.67
  pilT Vibrio cholerae strain A1552

70.279

95.28

0.67
  pilT Neisseria meningitidis 8013

65.97

98.82

0.652
  pilT Neisseria gonorrhoeae MS11

65.672

98.82

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.148

99.41

0.499
  pilU Pseudomonas stutzeri DSM 10701

41.033

97.05

0.398
  pilU Vibrio cholerae strain A1552

41.231

95.87

0.395
  pilU Acinetobacter baylyi ADP1

40.426

97.05

0.392