Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OFY13_RS11450 Genome accession   NZ_CP110188
Coordinates   2535132..2536169 (-) Length   345 a.a.
NCBI ID   WP_264879865.1    Uniprot ID   -
Organism   Vibrio cholerae strain E1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2530132..2541169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OFY13_RS11425 (OFY13_11425) - 2530616..2531179 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  OFY13_RS11430 (OFY13_11430) ruvX 2531260..2531682 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  OFY13_RS11435 (OFY13_11435) tyrS 2531707..2532987 (-) 1281 WP_001088153.1 tyrosine--tRNA ligase -
  OFY13_RS11440 (OFY13_11440) - 2533096..2533893 (-) 798 WP_001123224.1 LuxR C-terminal-related transcriptional regulator -
  OFY13_RS11445 (OFY13_11445) pilU 2534011..2535117 (-) 1107 WP_264879864.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OFY13_RS11450 (OFY13_11450) pilT 2535132..2536169 (-) 1038 WP_264879865.1 type IV pilus twitching motility protein PilT Machinery gene
  OFY13_RS11455 (OFY13_11455) - 2536195..2536905 (+) 711 WP_114730689.1 YggS family pyridoxal phosphate-dependent enzyme -
  OFY13_RS11460 (OFY13_11460) proC 2536958..2537776 (+) 819 WP_000437940.1 pyrroline-5-carboxylate reductase -
  OFY13_RS11465 (OFY13_11465) - 2537830..2538387 (+) 558 WP_001087261.1 YggT family protein -
  OFY13_RS11470 (OFY13_11470) yggU 2538387..2538677 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  OFY13_RS11475 (OFY13_11475) - 2538723..2539154 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  OFY13_RS11480 (OFY13_11480) - 2539224..2539826 (+) 603 WP_000725007.1 XTP/dITP diphosphatase -
  OFY13_RS11485 (OFY13_11485) hemW 2539820..2540995 (+) 1176 WP_000957329.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38132.69 Da        Isoelectric Point: 6.5570

>NTDB_id=752956 OFY13_RS11450 WP_264879865.1 2535132..2536169(-) (pilT) [Vibrio cholerae strain E1]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVGKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=752956 OFY13_RS11450 WP_264879865.1 2535132..2536169(-) (pilT) [Vibrio cholerae strain E1]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCCGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTGGGAAGCTTGGCGTACCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGTTAGA
GCAACTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGTT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTTTGGTTGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CGCTTACCGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTACACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTCTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGTGTCGGGGGTGGCCGTGTTGCGTGTCATGAAATCATGCTGGCAACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCGCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.71

100

0.997
  pilT Vibrio cholerae strain A1552

99.71

100

0.997
  pilT Acinetobacter baumannii D1279779

71.429

97.391

0.696
  pilT Acinetobacter baumannii strain A118

71.429

97.391

0.696
  pilT Acinetobacter nosocomialis M2

71.131

97.391

0.693
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377