NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	OH720_RS29940	Genome accession	NZ_CP110128
Coordinates	6641344..6642378 (-)	Length	344 a.a.
NCBI ID	WP_272603884.1	Uniprot ID	-
Organism	Pseudomonas sp. WJP1
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6636344..6647378

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH720_RS29910 (OH720_29920)	ruvX	6636505..6636942 (+)	438	WP_020797382.1	Holliday junction resolvase RuvX	-
OH720_RS29915 (OH720_29925)	pyrR	6637029..6637532 (+)	504	WP_008063485.1	bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR	-
OH720_RS29920 (OH720_29930)	-	6637560..6638564 (+)	1005	WP_008063486.1	aspartate carbamoyltransferase catalytic subunit	-
OH720_RS29925 (OH720_29935)	-	6638561..6639832 (+)	1272	WP_272603881.1	dihydroorotase	-
OH720_RS29930 (OH720_29940)	-	6639918..6640346 (-)	429	WP_272603882.1	TM2 domain-containing protein	-
OH720_RS29935 (OH720_29945)	-	6640596..6641213 (+)	618	WP_272603883.1	C40 family peptidase	-
OH720_RS29940 (OH720_29950)	pilT	6641344..6642378 (-)	1035	WP_272603884.1	type IV pilus twitching motility protein PilT	Machinery gene
OH720_RS29945 (OH720_29955)	-	6642435..6643121 (+)	687	WP_272603885.1	YggS family pyridoxal phosphate-dependent enzyme	-
OH720_RS29950 (OH720_29960)	proC	6643156..6643974 (+)	819	WP_272603886.1	pyrroline-5-carboxylate reductase	-
OH720_RS29955 (OH720_29965)	-	6643999..6644586 (+)	588	WP_272603887.1	YggT family protein	-
OH720_RS29960 (OH720_29970)	-	6644809..6645948 (+)	1140	WP_180202297.1	homoserine O-acetyltransferase	-
OH720_RS29965 (OH720_29975)	metW	6645956..6646576 (+)	621	WP_008063505.1	methionine biosynthesis protein MetW	-
OH720_RS29970 (OH720_29980)	-	6646602..6647036 (+)	435	WP_008063507.1	DUF4426 domain-containing protein	-

Sequence

Protein

Download Length: 344 a.a. Molecular weight: 37924.52 Da Isoelectric Point: 7.1232

                                >NTDB_id=752548 OH720_RS29940 WP_272603884.1 6641344..6642378(-) (pilT) [Pseudomonas sp. WJP1]

MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKEVQALIYDIMNDAQRVAFERHLETDFSFDVPGVAR

FRVNAFNQSRGAGAVFRTIPSRVQSMEELGMGEVFRKMTDAPRGLVLVTGPTGSGKSTTLAAMIDYLNSHRHHHILTIED

PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI

IDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSSIQAGGSLGMQTLDMGL

KELVTKGLISREQAREKARSPDNF

Nucleotide

Download Length: 1035 bp

                                >NTDB_id=752548 OH720_RS29940 WP_272603884.1 6641344..6642378(-) (pilT) [Pseudomonas sp. WJP1]

ATGGATATCACTGAGTTGCTGGCATTCAGCGCCAAACAAGGCGCATCCGACCTGCATTTGTCGGCAGGCCTGCCGCCGAT

GATTCGCGTCGATGGCGATGTGCGGCGCATCAACTTGCCGGCCCTGGACCACAAGGAAGTGCAGGCGCTGATCTACGACA

TCATGAACGACGCCCAGCGGGTCGCGTTCGAAAGGCACCTGGAAACCGACTTTTCCTTTGATGTGCCGGGTGTCGCGCGA

TTTCGGGTCAATGCCTTCAATCAGAGCCGAGGTGCCGGAGCGGTCTTTCGCACGATCCCGTCCAGGGTCCAGAGCATGGA

AGAGTTGGGCATGGGCGAAGTGTTTCGCAAGATGACCGACGCGCCACGCGGCCTGGTCCTGGTGACAGGGCCGACGGGGT

CCGGCAAGTCCACCACCCTGGCGGCGATGATCGATTACCTGAACAGCCATCGCCATCACCACATCCTCACAATCGAAGAC

CCTATCGAATTTGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCACCGCGATACCCGCAGTTTCGCCAC

AGCCCTGCGTTCGGCCCTGCGCGAAGACCCGGACGTGATCCTGGTGGGGGAGATGCGTGACCTGGAAACCATCCGCCTGG

CCTTGACCGCTGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCACCTCGGCGGCAAAAACCATCGACCGGATC

ATCGACGTGTTTCCGGGGGACGAGAAGTCGATGGTGCGTTCGATGTTGTCCGAGTCGTTGCTGGCGGTGGTGTCCCAGAC

CCTGGTGAAGAAGATTGGGGGAGGTCGGGTCGCGGCCCACGAGATCATGCTCGGAACGTCGGCGATCCGTAACCTGATCC

GGGAAGACAAGGTGGCACAAATGTACTCGTCGATTCAGGCGGGCGGGTCGCTGGGAATGCAGACGCTGGATATGGGCTTG

AAGGAGTTGGTGACCAAGGGCCTGATCAGTCGCGAGCAGGCGCGGGAGAAGGCGCGGTCGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Pseudomonas aeruginosa PAK	85.174	100	0.852
pilT	Pseudomonas stutzeri DSM 10701	85.174	100	0.852
pilT	Acinetobacter baylyi ADP1	77.616	100	0.776
pilT	Acinetobacter baumannii strain A118	77.035	100	0.77
pilT	Acinetobacter nosocomialis M2	77.035	100	0.77
pilT	Acinetobacter baumannii D1279779	77.035	100	0.77
pilT	Legionella pneumophila strain Lp02	72.674	100	0.727
pilT	Legionella pneumophila strain ERS1305867	72.674	100	0.727
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	68.546	97.965	0.672
pilT	Vibrio cholerae strain A1552	68.546	97.965	0.672
pilT	Neisseria meningitidis 8013	65.797	100	0.66
pilT	Neisseria gonorrhoeae MS11	65.507	100	0.657
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	51.304	100	0.515
pilU	Vibrio cholerae strain A1552	41.739	100	0.419
pilU	Pseudomonas stutzeri DSM 10701	40.87	100	0.41
pilU	Acinetobacter baylyi ADP1	40.173	100	0.404

Detailed information NTG0752548