Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N6N63_RS17745 Genome accession   NZ_CP110098
Coordinates   3726690..3727670 (-) Length   326 a.a.
NCBI ID   WP_223604697.1    Uniprot ID   -
Organism   Enterobacter asburiae strain GDFQ9D152     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3721690..3732670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N6N63_RS17715 (N6N63_17720) - 3722566..3723063 (+) 498 WP_032667932.1 SprT family zinc-dependent metalloprotease -
  N6N63_RS17720 (N6N63_17725) endA 3723158..3723865 (+) 708 WP_223604695.1 deoxyribonuclease I -
  N6N63_RS17725 (N6N63_17730) rsmE 3723917..3724648 (+) 732 WP_223604696.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N6N63_RS17730 (N6N63_17735) gshB 3724668..3725615 (+) 948 WP_029739222.1 glutathione synthase -
  N6N63_RS17735 (N6N63_17740) - 3725703..3726263 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  N6N63_RS17740 (N6N63_17745) ruvX 3726263..3726679 (+) 417 WP_059445426.1 Holliday junction resolvase RuvX -
  N6N63_RS17745 (N6N63_17750) pilT 3726690..3727670 (-) 981 WP_223604697.1 type IV pilus twitching motility protein PilT Machinery gene
  N6N63_RS17750 (N6N63_17755) - 3727688..3728389 (+) 702 WP_045403918.1 YggS family pyridoxal phosphate-dependent enzyme -
  N6N63_RS17755 (N6N63_17760) - 3728411..3728977 (+) 567 WP_023309130.1 YggT family protein -
  N6N63_RS17760 (N6N63_17765) yggU 3728974..3729270 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  N6N63_RS17765 (N6N63_17770) - 3729274..3729867 (+) 594 WP_059347697.1 XTP/dITP diphosphatase -
  N6N63_RS17770 (N6N63_17775) hemW 3729860..3731002 (+) 1143 WP_223604699.1 radical SAM family heme chaperone HemW -
  N6N63_RS17775 (N6N63_17780) - 3731066..3731404 (+) 339 WP_045888334.1 endonuclease domain-containing protein -
  N6N63_RS17780 (N6N63_17785) - 3731487..3732203 (-) 717 WP_023309135.1 DUF2884 domain-containing protein -
  N6N63_RS17785 (N6N63_17790) - 3732260..3732586 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35505.53 Da        Isoelectric Point: 6.9108

>NTDB_id=752199 N6N63_RS17745 WP_223604697.1 3726690..3727670(-) (pilT) [Enterobacter asburiae strain GDFQ9D152]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGIWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLGGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=752199 N6N63_RS17745 WP_223604697.1 3726690..3727670(-) (pilT) [Enterobacter asburiae strain GDFQ9D152]
ATGGATGTGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGTCGGCTTGAACCCGCTCCTTTTCCGCCTCCCGATGTCGGAGCGTTATTGAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATCTGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGCTGCCGCGAAGCTGCCCACAGCTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGTAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATTTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACACCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGGCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362