Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GN112_RS16665 Genome accession   NZ_AP021879
Coordinates   3762000..3763052 (+) Length   350 a.a.
NCBI ID   WP_155311247.1    Uniprot ID   A0A5K8ABX4
Organism   Desulfosarcina ovata subsp. ovata strain oXyS1     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3757000..3768052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GN112_RS16635 (DSCOOX_33360) - 3757618..3758364 (+) 747 WP_155311241.1 hypothetical protein -
  GN112_RS16640 (DSCOOX_33370) - 3758370..3759257 (+) 888 WP_155311242.1 hypothetical protein -
  GN112_RS16645 (DSCOOX_33380) yeiP 3759261..3759833 (-) 573 WP_155311243.1 elongation factor P-like protein YeiP -
  GN112_RS16650 (DSCOOX_33390) - 3760109..3760477 (+) 369 WP_155311244.1 hypothetical protein -
  GN112_RS16655 (DSCOOX_33400) - 3760599..3760850 (+) 252 WP_155311245.1 hypothetical protein -
  GN112_RS16660 (DSCOOX_33410) - 3760900..3761976 (+) 1077 WP_155311246.1 type IV pilus twitching motility protein PilT -
  GN112_RS16665 (DSCOOX_33420) pilT 3762000..3763052 (+) 1053 WP_155311247.1 type IV pilus twitching motility protein PilT Machinery gene
  GN112_RS16670 (DSCOOX_33430) - 3763063..3764064 (-) 1002 WP_155311248.1 GNAT family N-acetyltransferase -
  GN112_RS16675 (DSCOOX_33440) purB 3764111..3765463 (-) 1353 WP_155311249.1 adenylosuccinate lyase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38755.55 Da        Isoelectric Point: 5.3014

>NTDB_id=75199 GN112_RS16665 WP_155311247.1 3762000..3763052(+) (pilT) [Desulfosarcina ovata subsp. ovata strain oXyS1]
MATIDTYFKEMKQEGASDLHLAIGFPPMIRQSGELRPLEHPVITAEANRKILFEILTDEQQESVEENLDFDMAYELAGVG
RFRCNIFHQQRGFGAVFRIIPTTILTLEQLNLPEAVRSVADFKKGLVLVTGPTGSGKSTTLAAMINYINETRDAHIITIE
DPLEFVHPNKKCIFTQREIGTHAKSFADALKVAGREDPDVILVGEMRDLETISLALTCAELGILVFGTLHTNSAAKTIDR
IINAFPSNQQAQARTMLSESLRAVIAQQLLRTADGKGRCAANEILIGSSALASMIREGKITQIESLIQTGTKSGMQTMDQ
HLMQLVEEEKITREAAYEKAINKKLFEPLE

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=75199 GN112_RS16665 WP_155311247.1 3762000..3763052(+) (pilT) [Desulfosarcina ovata subsp. ovata strain oXyS1]
ATGGCAACGATCGACACCTATTTCAAAGAGATGAAGCAAGAAGGCGCCAGTGACCTGCATCTGGCCATCGGCTTTCCTCC
CATGATCCGCCAGAGCGGCGAACTGCGGCCGCTGGAACACCCGGTCATTACGGCCGAAGCCAACCGTAAGATTCTTTTCG
AAATCCTCACCGACGAGCAGCAGGAAAGCGTGGAGGAGAATCTGGACTTCGACATGGCCTATGAGCTGGCGGGGGTGGGG
CGTTTCCGCTGCAACATTTTTCACCAGCAGCGGGGCTTCGGCGCGGTGTTCCGGATCATTCCGACCACGATCCTGACCCT
GGAACAGCTGAACCTTCCCGAGGCGGTCCGGTCCGTCGCCGACTTCAAAAAGGGGTTGGTACTGGTCACCGGCCCCACGG
GCAGCGGCAAATCGACCACCCTGGCGGCCATGATCAATTATATCAACGAAACCCGGGACGCCCATATCATCACCATCGAA
GACCCCCTGGAGTTTGTACACCCCAATAAGAAATGCATCTTCACCCAGCGTGAAATCGGCACCCACGCCAAGAGCTTCGC
CGACGCGCTCAAGGTCGCCGGCCGGGAGGATCCGGACGTGATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCTCCC
TGGCCCTGACCTGTGCCGAGCTGGGGATTCTGGTTTTCGGCACCCTGCACACCAACAGCGCGGCCAAAACCATCGACCGC
ATCATCAACGCTTTTCCCTCGAACCAGCAGGCCCAGGCGCGCACCATGCTCTCCGAGTCCCTGAGGGCCGTCATCGCCCA
GCAACTGCTCCGCACCGCGGACGGCAAGGGGCGCTGCGCGGCCAATGAAATCCTCATCGGTTCGTCGGCCCTGGCCAGCA
TGATCCGGGAAGGCAAGATCACCCAGATCGAGTCACTGATTCAGACGGGAACCAAGTCGGGCATGCAGACCATGGATCAG
CATCTGATGCAACTGGTGGAAGAAGAGAAGATCACCCGCGAGGCGGCCTACGAAAAGGCGATCAATAAAAAGCTGTTCGA
GCCCCTGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K8ABX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

57.143

94

0.537
  pilT Neisseria meningitidis 8013

56.839

94

0.534
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

58.917

89.714

0.529
  pilT Vibrio cholerae strain A1552

58.917

89.714

0.529
  pilT Legionella pneumophila strain Lp02

54.985

94.571

0.52
  pilT Legionella pneumophila strain ERS1305867

54.985

94.571

0.52
  pilT Pseudomonas aeruginosa PAK

55.183

93.714

0.517
  pilT Pseudomonas stutzeri DSM 10701

54.878

93.714

0.514
  pilT Acinetobacter baumannii D1279779

53.593

95.429

0.511
  pilT Acinetobacter nosocomialis M2

53.593

95.429

0.511
  pilT Acinetobacter baumannii strain A118

53.593

95.429

0.511
  pilT Acinetobacter baylyi ADP1

57.006

89.714

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.298

96

0.483
  pilU Vibrio cholerae strain A1552

42.433

96.286

0.409
  pilU Pseudomonas stutzeri DSM 10701

43.558

93.143

0.406
  pilU Acinetobacter baylyi ADP1

39.879

94.571

0.377

Multiple sequence alignment