Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GN113_RS21280 Genome accession   NZ_AP021876
Coordinates   4876861..4877913 (+) Length   350 a.a.
NCBI ID   WP_155323863.1    Uniprot ID   A0A5K7ZU46
Organism   Desulfosarcina ovata subsp. sediminis strain 28bB2T     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4871861..4882913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GN113_RS21250 (DSCO28_42800) - 4872480..4873226 (+) 747 WP_155323858.1 hypothetical protein -
  GN113_RS21255 (DSCO28_42810) - 4873232..4874119 (+) 888 WP_155323859.1 hypothetical protein -
  GN113_RS21260 (DSCO28_42820) yeiP 4874123..4874695 (-) 573 WP_155311243.1 elongation factor P-like protein YeiP -
  GN113_RS21265 (DSCO28_42830) - 4874970..4875338 (+) 369 WP_155323860.1 hypothetical protein -
  GN113_RS21270 (DSCO28_42840) - 4875460..4875711 (+) 252 WP_155323861.1 hypothetical protein -
  GN113_RS21275 (DSCO28_42850) - 4875761..4876837 (+) 1077 WP_155323862.1 type IV pilus twitching motility protein PilT -
  GN113_RS21280 (DSCO28_42860) pilT 4876861..4877913 (+) 1053 WP_155323863.1 type IV pilus twitching motility protein PilT Machinery gene
  GN113_RS21285 (DSCO28_42870) - 4877924..4878925 (-) 1002 WP_155323864.1 GNAT family N-acetyltransferase -
  GN113_RS21290 (DSCO28_42880) purB 4878972..4880324 (-) 1353 WP_155323865.1 adenylosuccinate lyase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38696.42 Da        Isoelectric Point: 5.4630

>NTDB_id=75188 GN113_RS21280 WP_155323863.1 4876861..4877913(+) (pilT) [Desulfosarcina ovata subsp. sediminis strain 28bB2T]
MATIDTYFKEMKQNGASDLHLAIGFPPMIRQSGELRPLDHPVITAEANRKILYEILTDEQQESVEENLDFDMAYELAGVG
RFRCNIFHQQRGFGAVFRIIPTTILTLEQLNLPEAVRSIADFKKGLVLVTGPTGSGKSTTLAAMINYINETRDAHIITIE
DPLEFVHPNKKCIFTQREIGTHAKSFADALKVAGREDPDVILVGEMRDLETISLALTCAELGILVFGTLHTNSAAKTIDR
IINAFPSNQQAQARTMLSESLRAVIAQQLLRTADGKGRCAANEILIGSSALASMIREGKITQIESLIQTGTKSGMQTMDQ
HLAQLVEEEKITREAAYEKAINKKLFEPLE

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=75188 GN113_RS21280 WP_155323863.1 4876861..4877913(+) (pilT) [Desulfosarcina ovata subsp. sediminis strain 28bB2T]
ATGGCAACGATCGATACCTATTTCAAAGAGATGAAACAAAACGGCGCCAGTGACCTGCATCTGGCCATCGGCTTTCCCCC
CATGATCCGCCAGAGCGGTGAACTGCGGCCGCTGGATCACCCGGTCATTACGGCCGAAGCCAACCGAAAGATTCTTTACG
AGATCCTCACCGACGAGCAGCAGGAAAGCGTGGAAGAGAATCTGGACTTCGACATGGCCTATGAGCTCGCGGGGGTGGGG
CGTTTCCGCTGCAACATTTTTCACCAGCAGCGGGGCTTCGGCGCCGTGTTCCGGATCATTCCGACCACGATCCTGACCCT
GGAACAGCTGAACCTTCCCGAGGCGGTCCGGTCCATCGCCGACTTCAAAAAGGGGTTGGTACTGGTCACCGGCCCCACGG
GCAGCGGCAAATCGACCACCCTGGCGGCCATGATCAACTATATCAACGAAACCCGGGACGCCCATATCATCACCATCGAA
GACCCCCTGGAGTTTGTACACCCCAATAAAAAATGCATCTTCACCCAGCGTGAAATCGGCACCCACGCCAAGAGCTTCGC
CGACGCGCTCAAGGTCGCCGGCCGGGAGGATCCGGACGTGATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCTCCC
TGGCCCTGACCTGTGCCGAGCTGGGGATTCTGGTTTTCGGCACCCTGCACACCAACAGCGCGGCCAAAACCATCGACCGC
ATCATCAACGCCTTTCCCTCGAACCAGCAGGCCCAGGCGCGCACCATGCTCTCCGAGTCCCTGAGAGCCGTCATCGCCCA
GCAACTGCTCCGCACCGCAGACGGCAAGGGGCGCTGCGCGGCCAATGAGATCCTCATCGGTTCGTCGGCCCTGGCCAGCA
TGATCCGTGAAGGCAAAATCACCCAGATCGAGTCGCTGATTCAGACCGGAACCAAATCGGGCATGCAGACCATGGATCAG
CATCTGGCCCAACTGGTGGAAGAAGAAAAAATCACCCGCGAGGCGGCCTACGAAAAGGCGATCAATAAAAAACTGTTCGA
ACCCCTGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K7ZU46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

57.229

94.857

0.543
  pilT Neisseria meningitidis 8013

56.928

94.857

0.54
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

58.917

89.714

0.529
  pilT Vibrio cholerae strain A1552

58.917

89.714

0.529
  pilT Legionella pneumophila strain Lp02

55.09

95.429

0.526
  pilT Legionella pneumophila strain ERS1305867

55.09

95.429

0.526
  pilT Acinetobacter baylyi ADP1

57.962

89.714

0.52
  pilT Pseudomonas stutzeri DSM 10701

55.488

93.714

0.52
  pilT Acinetobacter baumannii strain A118

54.491

95.429

0.52
  pilT Acinetobacter nosocomialis M2

54.491

95.429

0.52
  pilT Acinetobacter baumannii D1279779

54.491

95.429

0.52
  pilT Pseudomonas aeruginosa PAK

55.183

93.714

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.343

95.714

0.491
  pilU Vibrio cholerae strain A1552

42.433

96.286

0.409
  pilU Pseudomonas stutzeri DSM 10701

43.558

93.143

0.406
  pilU Acinetobacter baylyi ADP1

39.879

94.571

0.377

Multiple sequence alignment