Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DSCA_RS27545 Genome accession   NZ_AP021874
Coordinates   6344542..6345624 (+) Length   360 a.a.
NCBI ID   WP_155319497.1    Uniprot ID   -
Organism   Desulfosarcina alkanivorans strain PL12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6339542..6350624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSCA_RS27520 (DSCA_56250) - 6339857..6341011 (+) 1155 WP_155319492.1 FAD-binding oxidoreductase -
  DSCA_RS27525 (DSCA_56260) - 6341048..6343186 (+) 2139 WP_155319493.1 aldehyde ferredoxin oxidoreductase family protein -
  DSCA_RS27530 (DSCA_56270) - 6343183..6343380 (+) 198 WP_155319494.1 MoaD/ThiS family protein -
  DSCA_RS27535 (DSCA_56280) - 6343618..6343917 (+) 300 WP_155319495.1 YggT family protein -
  DSCA_RS27540 (DSCA_56290) - 6343943..6344440 (+) 498 WP_155319496.1 DivIVA domain-containing protein -
  DSCA_RS27545 (DSCA_56300) pilT 6344542..6345624 (+) 1083 WP_155319497.1 type IV pilus twitching motility protein PilT Machinery gene
  DSCA_RS27550 (DSCA_56310) - 6345671..6346834 (+) 1164 WP_155319498.1 type IV pilus twitching motility protein PilT -
  DSCA_RS27555 (DSCA_56320) - 6346884..6347540 (+) 657 WP_155319499.1 zinc-ribbon domain-containing protein -
  DSCA_RS27560 (DSCA_56330) - 6347585..6347743 (+) 159 WP_155319500.1 hypothetical protein -
  DSCA_RS27565 (DSCA_56340) - 6347746..6348687 (+) 942 WP_155319501.1 MBL fold metallo-hydrolase -
  DSCA_RS27570 (DSCA_56350) - 6348752..6349519 (-) 768 WP_155319502.1 type III pantothenate kinase -
  DSCA_RS27575 (DSCA_56360) - 6349533..6350462 (-) 930 WP_155319503.1 CdaR family protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39892.91 Da        Isoelectric Point: 8.1231

>NTDB_id=75169 DSCA_RS27545 WP_155319497.1 6344542..6345624(+) (pilT) [Desulfosarcina alkanivorans strain PL12]
MAKIDAFFKLMHEQNASDLHLLSGQPPALRIHGDIERVKYKVLDNDDLRKMLYEITPEDKVKSFEETGDVDFGYEIPGLA
RYRANYFMQKNGIGAVFREIPSSIMTAEQLGLPPVISKLASLPRGLVIVTGPTGSGKSTTLAAIIDVANRTRSDHIITVE
DPIEFVHKSRSCLVNHREVGLHTKSFSAALRGALREDPDIILVGEMRDLETISLAIEASATGHLVFGTLHTTSAPKTVDR
IVEVFPAQEQAQIRSTLSDGIRAIISQTLFKRIDKKGRVPAMEILIATPAVRNLIRESKSHQLPSMMQTGKKYGMQLLDD
AIMDLYNKGRISADDAYTKANDKGRFRPLLKSPPTDFTEA

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=75169 DSCA_RS27545 WP_155319497.1 6344542..6345624(+) (pilT) [Desulfosarcina alkanivorans strain PL12]
ATGGCCAAGATTGATGCTTTTTTCAAGCTCATGCACGAACAAAATGCCTCCGACCTCCACCTGCTGTCCGGTCAGCCGCC
CGCGCTGCGCATCCATGGCGACATCGAGCGGGTGAAATACAAGGTGCTGGACAACGATGATCTTCGCAAGATGCTCTACG
AGATCACTCCGGAAGACAAGGTCAAGAGCTTCGAGGAGACCGGCGATGTGGACTTCGGCTACGAGATCCCCGGCCTGGCC
CGCTACCGGGCCAACTACTTCATGCAGAAGAACGGTATCGGGGCGGTATTCCGGGAAATTCCCAGCAGCATCATGACCGC
CGAACAGCTCGGCCTGCCTCCGGTGATCTCCAAGCTGGCCTCGCTGCCCCGGGGACTGGTTATCGTCACCGGCCCCACCG
GTTCGGGTAAATCCACCACCCTGGCGGCCATTATCGATGTGGCCAACCGCACCCGCAGCGATCACATCATCACCGTAGAA
GACCCGATCGAATTTGTGCATAAAAGCCGGAGTTGCCTGGTGAACCACCGGGAGGTGGGCCTGCATACCAAATCCTTCAG
TGCGGCCCTGCGCGGCGCCCTTCGCGAGGATCCGGACATCATCCTGGTCGGTGAAATGCGGGACCTGGAAACCATCTCCC
TGGCCATCGAGGCCAGTGCCACGGGGCACCTGGTATTCGGCACCCTGCACACCACCAGTGCACCCAAAACGGTGGACCGT
ATCGTTGAGGTCTTCCCCGCCCAGGAGCAGGCCCAGATCCGCTCCACCCTGTCCGACGGTATCCGGGCCATCATTTCCCA
GACACTGTTCAAGCGCATCGACAAGAAGGGTCGTGTGCCGGCCATGGAAATTCTCATCGCCACACCGGCCGTGCGCAACC
TGATTCGTGAATCCAAATCCCACCAGCTGCCCTCCATGATGCAGACGGGCAAAAAATACGGCATGCAGCTTCTTGACGAC
GCCATCATGGACCTTTACAACAAGGGGCGGATCAGTGCCGACGATGCCTACACCAAAGCCAACGACAAGGGGCGCTTCCG
GCCGCTGCTTAAATCGCCGCCAACCGATTTTACCGAAGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

57.186

92.778

0.531
  pilT Pseudomonas aeruginosa PAK

57.317

91.111

0.522
  pilT Legionella pneumophila strain Lp02

54.79

92.778

0.508
  pilT Legionella pneumophila strain ERS1305867

54.79

92.778

0.508
  pilT Acinetobacter nosocomialis M2

53.293

92.778

0.494
  pilT Acinetobacter baumannii D1279779

53.293

92.778

0.494
  pilT Acinetobacter baumannii strain A118

53.293

92.778

0.494
  pilT Acinetobacter baylyi ADP1

53.293

92.778

0.494
  pilT Neisseria meningitidis 8013

52.905

90.833

0.481
  pilT Neisseria gonorrhoeae MS11

52.599

90.833

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

90.833

0.464
  pilT Vibrio cholerae strain A1552

51.07

90.833

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.522

95.278

0.453
  pilU Pseudomonas stutzeri DSM 10701

39.542

96.944

0.383
  pilU Acinetobacter baylyi ADP1

38.439

96.111

0.369
  pilU Vibrio cholerae strain A1552

37.006

98.333

0.364

Multiple sequence alignment