Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OK026_RS14265 Genome accession   NZ_CP109905
Coordinates   3137750..3138787 (+) Length   345 a.a.
NCBI ID   WP_043639309.1    Uniprot ID   A0A1W0CSM2
Organism   Chromobacterium haemolyticum strain UGAL515B_03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3132750..3143787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK026_RS14235 (OK026_14255) aroG 3132961..3134022 (-) 1062 WP_019102527.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  OK026_RS14240 (OK026_14260) dksA 3134254..3134664 (-) 411 WP_019102526.1 RNA polymerase-binding protein DksA -
  OK026_RS14245 (OK026_14265) - 3134949..3135254 (-) 306 WP_019102525.1 c-type cytochrome -
  OK026_RS14250 (OK026_14270) - 3135346..3135927 (-) 582 WP_043589318.1 YggT family protein -
  OK026_RS14255 (OK026_14275) proC 3135936..3136739 (-) 804 WP_043639303.1 pyrroline-5-carboxylate reductase -
  OK026_RS14260 (OK026_14280) - 3136793..3137484 (-) 692 Protein_2792 YggS family pyridoxal phosphate-dependent enzyme -
  OK026_RS14265 (OK026_14285) pilT 3137750..3138787 (+) 1038 WP_043639309.1 type IV pilus twitching motility protein PilT Machinery gene
  OK026_RS14270 (OK026_14290) pilU 3138833..3139969 (+) 1137 WP_043639312.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OK026_RS14275 (OK026_14295) - 3140100..3140687 (-) 588 WP_043639315.1 TetR/AcrR family transcriptional regulator -
  OK026_RS14280 (OK026_14300) - 3141146..3143065 (+) 1920 WP_052370646.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38350.14 Da        Isoelectric Point: 6.7774

>NTDB_id=750496 OK026_RS14265 WP_043639309.1 3137750..3138787(+) (pilT) [Chromobacterium haemolyticum strain UGAL515B_03]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDIRRINLPPMDHHDVHDMVYDIMNDYQRKIFEDTFECDFSFDLPGIAR
FRVNSFIQNRGMGAVFRVIPSKVLSLEQLGAPKIFQEIAAFPRGLVLVTGPTGSGKSTTLAAMIDFINDNHFSHILTVED
PIEFVHDSKKCLINQRELGLQTHSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVIAQTLLKTKDGSGRVAAHEIMLGTPAIRNLIRENKIAQINSMIQTGQQHGMQTLDQC
LQELVRRNMVSPADARQKAANKDQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=750496 OK026_RS14265 WP_043639309.1 3137750..3138787(+) (pilT) [Chromobacterium haemolyticum strain UGAL515B_03]
ATGGAAATTTCGGAGCTCTTGGCCTTTACGGTCAAGAACAAGGCGTCCGACCTGCACTTGTCCGCCGGGCTGCCGCCGAT
GATCCGGGTGAACGGCGACATCCGTCGCATCAACCTGCCGCCGATGGACCACCATGACGTGCACGACATGGTGTACGACA
TCATGAACGATTACCAGCGCAAAATCTTCGAAGACACCTTCGAGTGCGACTTTTCCTTCGATCTGCCCGGCATCGCCCGT
TTCCGCGTCAACTCATTCATACAGAACCGCGGCATGGGCGCGGTGTTTCGGGTGATTCCGTCCAAGGTGTTATCGCTGGA
ACAGCTGGGCGCGCCCAAGATCTTCCAGGAAATCGCCGCCTTTCCGCGCGGGCTGGTGCTGGTGACCGGCCCCACCGGCT
CCGGCAAATCCACTACGCTGGCGGCGATGATAGATTTCATCAACGACAACCACTTCTCCCACATCCTGACTGTCGAGGAC
CCGATCGAGTTTGTCCACGACAGCAAGAAGTGCCTGATCAACCAGCGTGAACTGGGTTTGCAGACACACAGCTTCGCCAA
TGCCTTGAAGTCCGCCTTGCGGGAAGACCCGGATGTGATCCTGGTGGGCGAACTGCGCGATCTGGAAACCATACGCCTGG
CTTTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCGGCCAAGACCATAGACCGCATC
GTCGACGTGTTCCCGGCCGGGGAAAAGGAGATGGTGCGCTCCATGCTGTCCGAGTCGGTGCGCGCGGTGATCGCCCAGAC
CCTGCTCAAAACCAAGGACGGCAGCGGGCGGGTGGCGGCGCATGAAATCATGCTGGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGATTGCTCAAATCAACTCCATGATCCAGACCGGCCAGCAGCATGGGATGCAGACGCTGGACCAGTGC
CTGCAGGAGCTGGTGCGGCGCAATATGGTGTCGCCGGCGGACGCCAGGCAAAAGGCCGCCAACAAGGATCAATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W0CSM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

72.174

100

0.722
  pilT Acinetobacter baumannii strain A118

72.174

100

0.722
  pilT Acinetobacter baumannii D1279779

72.174

100

0.722
  pilT Neisseria meningitidis 8013

71.429

99.42

0.71
  pilT Neisseria gonorrhoeae MS11

71.429

99.42

0.71
  pilT Pseudomonas stutzeri DSM 10701

71.014

100

0.71
  pilT Legionella pneumophila strain Lp02

70.725

100

0.707
  pilT Pseudomonas aeruginosa PAK

70.725

100

0.707
  pilT Legionella pneumophila strain ERS1305867

70.725

100

0.707
  pilT Acinetobacter baylyi ADP1

70.435

100

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.568

97.971

0.652
  pilT Vibrio cholerae strain A1552

66.568

97.971

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.888

98.261

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406