Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OHY99_RS21625 Genome accession   NZ_CP109824
Coordinates   4465530..4466552 (-) Length   340 a.a.
NCBI ID   WP_043141339.1    Uniprot ID   -
Organism   Serratia marcescens strain SMBC31     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4460530..4471552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHY99_RS21595 (OHY99_21600) - 4461329..4461841 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  OHY99_RS21600 (OHY99_21605) endA 4461943..4462638 (+) 696 WP_089196263.1 deoxyribonuclease I -
  OHY99_RS21605 (OHY99_21610) rsmE 4462708..4463439 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OHY99_RS21610 (OHY99_21615) gshB 4463450..4464400 (+) 951 WP_043141334.1 glutathione synthase -
  OHY99_RS21615 (OHY99_21620) - 4464548..4465111 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  OHY99_RS21620 (OHY99_21625) ruvX 4465111..4465533 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  OHY99_RS21625 (OHY99_21630) pilT 4465530..4466552 (-) 1023 WP_043141339.1 type IV pilus twitching motility protein PilT Machinery gene
  OHY99_RS21630 (OHY99_21635) - 4466573..4467280 (+) 708 WP_197836019.1 YggS family pyridoxal phosphate-dependent enzyme -
  OHY99_RS21635 (OHY99_21640) proC 4467300..4468121 (+) 822 WP_049296238.1 pyrroline-5-carboxylate reductase -
  OHY99_RS21640 (OHY99_21645) - 4468153..4468707 (+) 555 WP_025304250.1 YggT family protein -
  OHY99_RS21645 (OHY99_21650) yggU 4468704..4468994 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  OHY99_RS21650 (OHY99_21655) - 4469012..4469605 (+) 594 WP_197836021.1 XTP/dITP diphosphatase -
  OHY99_RS21655 (OHY99_21660) hemW 4469598..4470740 (+) 1143 WP_164064284.1 radical SAM family heme chaperone HemW -
  OHY99_RS21660 (OHY99_21665) - 4470778..4471026 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  OHY99_RS21665 (OHY99_21670) - 4471010..4471252 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36559.78 Da        Isoelectric Point: 7.2889

>NTDB_id=749644 OHY99_RS21625 WP_043141339.1 4465530..4466552(-) (pilT) [Serratia marcescens strain SMBC31]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAQTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANIFQQSAGMSLALRRIARQSPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=749644 OHY99_RS21625 WP_043141339.1 4465530..4466552(-) (pilT) [Serratia marcescens strain SMBC31]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCAAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACATTTTTCAACAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGTATTGCCAGGCAATCGCCTTCGCT
TGCCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCAGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCTGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Pseudomonas aeruginosa PAK

49.254

98.529

0.485
  pilT Neisseria gonorrhoeae MS11

49.254

98.529

0.485
  pilT Neisseria meningitidis 8013

49.849

97.353

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.353

0.476
  pilT Legionella pneumophila strain ERS1305867

48.943

97.353

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368