Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OI909_RS16760 Genome accession   NZ_CP109694
Coordinates   3432327..3433307 (-) Length   326 a.a.
NCBI ID   WP_284592899.1    Uniprot ID   -
Organism   Enterobacter asburiae strain 2017-45-54-05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3427327..3438307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OI909_RS16730 (OI909_16725) - 3428203..3428700 (+) 498 WP_033146604.1 SprT family zinc-dependent metalloprotease -
  OI909_RS16735 (OI909_16730) endA 3428795..3429502 (+) 708 WP_023333343.1 deoxyribonuclease I -
  OI909_RS16740 (OI909_16735) rsmE 3429554..3430285 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OI909_RS16745 (OI909_16740) gshB 3430305..3431252 (+) 948 WP_029739222.1 glutathione synthase -
  OI909_RS16750 (OI909_16745) - 3431340..3431900 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  OI909_RS16755 (OI909_16750) ruvX 3431900..3432316 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  OI909_RS16760 (OI909_16755) pilT 3432327..3433307 (-) 981 WP_284592899.1 type IV pilus twitching motility protein PilT Machinery gene
  OI909_RS16765 (OI909_16760) - 3433325..3434029 (+) 705 WP_284592900.1 YggS family pyridoxal phosphate-dependent enzyme -
  OI909_RS16770 (OI909_16765) - 3434048..3434614 (+) 567 WP_284592901.1 YggT family protein -
  OI909_RS16775 (OI909_16770) yggU 3434611..3434907 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  OI909_RS16780 (OI909_16775) - 3434911..3435504 (+) 594 WP_045403921.1 XTP/dITP diphosphatase -
  OI909_RS16785 (OI909_16780) hemW 3435497..3436633 (+) 1137 WP_284592902.1 radical SAM family heme chaperone HemW -
  OI909_RS16790 (OI909_16785) - 3436703..3437041 (+) 339 WP_039262962.1 endonuclease domain-containing protein -
  OI909_RS16795 (OI909_16790) - 3437124..3437840 (-) 717 WP_024906551.1 DUF2884 domain-containing protein -
  OI909_RS16800 (OI909_16795) - 3437897..3438223 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35484.51 Da        Isoelectric Point: 6.8406

>NTDB_id=748873 OI909_RS16760 WP_284592899.1 3432327..3433307(-) (pilT) [Enterobacter asburiae strain 2017-45-54-05]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFQPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSLTLRLLPRSCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQPSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=748873 OI909_RS16760 WP_284592899.1 3432327..3433307(-) (pilT) [Enterobacter asburiae strain 2017-45-54-05]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTGACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTTTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAACTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTAAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGACAGCCCAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAAAGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGCGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362