Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FYK59_RS10110 Genome accession   NZ_AP019853
Coordinates   1940736..1941779 (+) Length   347 a.a.
NCBI ID   WP_071200551.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain SS3160     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1935736..1946779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYK59_RS10085 - 1937831..1938103 (-) 273 WP_162855536.1 hypothetical protein -
  FYK59_RS10090 (NGSS3160_19470) dksA 1938118..1938534 (-) 417 WP_003692108.1 RNA polymerase-binding protein DksA -
  FYK59_RS10095 (NGSS3160_19480) proC 1938683..1939474 (-) 792 WP_103195364.1 pyrroline-5-carboxylate reductase -
  FYK59_RS10100 (NGSS3160_19490) - 1939523..1939930 (-) 408 WP_003701818.1 hypothetical protein -
  FYK59_RS10105 (NGSS3160_19500) - 1939930..1940616 (-) 687 WP_003690201.1 YggS family pyridoxal phosphate-dependent enzyme -
  FYK59_RS10110 (NGSS3160_19510) pilT 1940736..1941779 (+) 1044 WP_071200551.1 type IV pilus twitching motility protein PilT Machinery gene
  FYK59_RS10115 (NGSS3160_19520) - 1941943..1943169 (+) 1227 WP_003697786.1 PilT/PilU family type 4a pilus ATPase -
  FYK59_RS10120 (NGSS3160_19530) yccS 1943286..1945436 (+) 2151 WP_003697785.1 YccS family putative transporter -
  FYK59_RS10125 (NGSS3160_19540) - 1945916..1946200 (-) 285 WP_003692093.1 GIY-YIG nuclease family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37912.26 Da        Isoelectric Point: 6.7304

>NTDB_id=74456 FYK59_RS10110 WP_071200551.1 1940736..1941779(+) (pilT) [Neisseria gonorrhoeae strain SS3160]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMQRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEAARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=74456 FYK59_RS10110 WP_071200551.1 1940736..1941779(+) (pilT) [Neisseria gonorrhoeae strain SS3160]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGATATGCAGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGCAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACACCGGCCGCGGCCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAACGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGAGTTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGAGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCGGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCCGCCGGAGAGAAAGAAATGGTGCGTTCCATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACGATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGCCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

99.712

100

0.997
  pilT Neisseria meningitidis 8013

98.847

100

0.988
  pilT Acinetobacter baumannii D1279779

67.536

99.424

0.671
  pilT Acinetobacter baumannii strain A118

67.536

99.424

0.671
  pilT Acinetobacter nosocomialis M2

67.536

99.424

0.671
  pilT Acinetobacter baylyi ADP1

66.957

99.424

0.666
  pilT Pseudomonas stutzeri DSM 10701

66.667

99.424

0.663
  pilT Pseudomonas aeruginosa PAK

66.087

99.424

0.657
  pilT Legionella pneumophila strain Lp02

64.223

98.271

0.631
  pilT Legionella pneumophila strain ERS1305867

64.223

98.271

0.631
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.939

95.101

0.608
  pilT Vibrio cholerae strain A1552

63.939

95.101

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

97.695

0.522
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401
  pilU Vibrio cholerae strain A1552

38.329

100

0.383
  pilU Acinetobacter baylyi ADP1

38.439

99.712

0.383

Multiple sequence alignment