Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QDY55_RS04525 Genome accession   NZ_CP122768
Coordinates   934687..935667 (+) Length   326 a.a.
NCBI ID   WP_074398146.1    Uniprot ID   -
Organism   Escherichia coli strain ETEC1738     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 929687..940667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDY55_RS04495 (QDY55_04490) - 930063..931049 (-) 987 WP_262145701.1 TRAP transporter substrate-binding protein -
  QDY55_RS04500 (QDY55_04495) hemW 931364..932500 (-) 1137 WP_001521257.1 radical SAM family heme chaperone HemW -
  QDY55_RS04505 (QDY55_04500) rdgB 932493..933086 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  QDY55_RS04510 (QDY55_04505) yggU 933094..933384 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  QDY55_RS04515 (QDY55_04510) yggT 933381..933947 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  QDY55_RS04520 (QDY55_04515) yggS 933965..934669 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  QDY55_RS04525 (QDY55_04520) pilT 934687..935667 (+) 981 WP_074398146.1 type IV pilus twitching motility protein PilT Machinery gene
  QDY55_RS04530 (QDY55_04525) ruvX 935782..936198 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  QDY55_RS04535 (QDY55_04530) yqgE 936198..936761 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  QDY55_RS04540 (QDY55_04535) gshB 936870..937820 (-) 951 WP_000593273.1 glutathione synthase -
  QDY55_RS04545 (QDY55_04540) rsmE 937833..938564 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QDY55_RS04550 (QDY55_04545) endA 938644..939351 (-) 708 WP_000286497.1 deoxyribonuclease I -
  QDY55_RS04555 (QDY55_04550) yggI 939446..939943 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35928.10 Da        Isoelectric Point: 5.7980

>NTDB_id=742907 QDY55_RS04525 WP_074398146.1 934687..935667(+) (pilT) [Escherichia coli strain ETEC1738]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSEAIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=742907 QDY55_RS04525 WP_074398146.1 934687..935667(+) (pilT) [Escherichia coli strain ETEC1738]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGGCAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGTTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362