Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QDW58_RS03955 Genome accession   NZ_CP122629
Coordinates   816129..817109 (+) Length   326 a.a.
NCBI ID   WP_282834303.1    Uniprot ID   -
Organism   Escherichia coli strain ETEC4088     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 811129..822109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDW58_RS03925 (QDW58_03925) yggM 811644..812651 (+) 1008 WP_000745217.1 DUF1202 family protein -
  QDW58_RS03930 (QDW58_03930) hemW 812806..813942 (-) 1137 WP_000239944.1 radical SAM family heme chaperone HemW -
  QDW58_RS03935 (QDW58_03935) rdgB 813935..814528 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  QDW58_RS03940 (QDW58_03940) yggU 814536..814826 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  QDW58_RS03945 (QDW58_03945) yggT 814823..815389 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  QDW58_RS03950 (QDW58_03950) yggS 815407..816111 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  QDW58_RS03955 (QDW58_03955) pilT 816129..817109 (+) 981 WP_282834303.1 type IV pilus twitching motility protein PilT Machinery gene
  QDW58_RS03960 (QDW58_03960) ruvX 817285..817701 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  QDW58_RS03965 (QDW58_03965) yqgE 817701..818264 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  QDW58_RS03970 (QDW58_03970) gshB 818373..819323 (-) 951 WP_000593273.1 glutathione synthase -
  QDW58_RS03975 (QDW58_03975) rsmE 819336..820067 (-) 732 WP_098723145.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QDW58_RS03980 (QDW58_03980) endA 820147..820854 (-) 708 WP_000286500.1 deoxyribonuclease I -
  QDW58_RS03985 (QDW58_03985) yggI 820949..821446 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35888.11 Da        Isoelectric Point: 5.5860

>NTDB_id=741593 QDW58_RS03955 WP_282834303.1 816129..817109(+) (pilT) [Escherichia coli strain ETEC4088]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDLRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=741593 QDW58_RS03955 WP_282834303.1 816129..817109(+) (pilT) [Escherichia coli strain ETEC4088]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCTGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(64-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368