Detailed information NTG0741190

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: QDX83_RS04150
• Genome accession: NZ_CP122600
• Coordinates: 867865..868845 (+)
• Length: 326 a.a.
• NCBI ID: WP_282832931.1
• Uniprot ID: -
• Organism: Escherichia coli strain ETEC6333
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 862865..873845

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QDX83_RS04120 (QDX83_04120)	-	863242..864228 (-)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-
QDX83_RS04125 (QDX83_04125)	hemW	864542..865678 (-)	1137	WP_063085753.1	radical SAM family heme chaperone HemW	-
QDX83_RS04130 (QDX83_04130)	rdgB	865671..866264 (-)	594	WP_001174747.1	XTP/dITP diphosphatase	-
QDX83_RS04135 (QDX83_04135)	yggU	866272..866562 (-)	291	WP_001277223.1	DUF167 family protein YggU	-
QDX83_RS04140 (QDX83_04140)	yggT	866559..867125 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
QDX83_RS04145 (QDX83_04145)	yggS	867143..867847 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
QDX83_RS04150 (QDX83_04150)	pilT	867865..868845 (+)	981	WP_282832931.1	type IV pilus twitching motility protein PilT	Machinery gene
QDX83_RS04155 (QDX83_04155)	ruvX	868960..869376 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
QDX83_RS04160 (QDX83_04160)	yqgE	869376..869939 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
QDX83_RS04165 (QDX83_04165)	gshB	870048..870998 (-)	951	WP_000593273.1	glutathione synthase	-
QDX83_RS04170 (QDX83_04170)	rsmE	871011..871742 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
QDX83_RS04175 (QDX83_04175)	endA	871822..872529 (-)	708	WP_000286500.1	deoxyribonuclease I	-
QDX83_RS04180 (QDX83_04180)	yggI	872624..873121 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35948.08 Da
• Isoelectric Point: 5.7980

>NTDB_id=741190 QDX83_RS04150 WP_282832931.1 867865..868845(+) (pilT) [Escherichia coli strain ETEC6333]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGASPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=741190 QDX83_RS04150 WP_282832931.1 867865..868845(+) (pilT) [Escherichia coli strain ETEC6333]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGTCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTGGGAGAGCTGCGTGACAGCGAAACGATACGTCTGGCGCTGACGG
CGGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.171	100	0.485
pilT	Neisseria gonorrhoeae MS11	47.866	100	0.482
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.063	97.239	0.39
pilU	Pseudomonas stutzeri DSM 10701	36.97	100	0.374
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362