Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QDY30_RS04405 Genome accession   NZ_CP122588
Coordinates   900110..901090 (+) Length   326 a.a.
NCBI ID   WP_071777673.1    Uniprot ID   -
Organism   Escherichia coli strain ETEC6335     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 895110..906090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDY30_RS04375 (QDY30_04375) yggM 895537..896544 (+) 1008 WP_053889673.1 DUF1202 family protein -
  QDY30_RS04380 (QDY30_04380) hemW 896787..897923 (-) 1137 WP_119457709.1 radical SAM family heme chaperone HemW -
  QDY30_RS04385 (QDY30_04385) rdgB 897916..898509 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  QDY30_RS04390 (QDY30_04390) yggU 898517..898807 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  QDY30_RS04395 (QDY30_04395) yggT 898804..899370 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  QDY30_RS04400 (QDY30_04400) yggS 899388..900092 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  QDY30_RS04405 (QDY30_04405) pilT 900110..901090 (+) 981 WP_071777673.1 type IV pilus twitching motility protein PilT Machinery gene
  QDY30_RS04410 (QDY30_04410) ruvX 901281..901697 (-) 417 WP_000017103.1 Holliday junction resolvase RuvX -
  QDY30_RS04415 (QDY30_04415) yqgE 901697..902260 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  QDY30_RS04420 (QDY30_04420) gshB 902369..903319 (-) 951 WP_000593273.1 glutathione synthase -
  QDY30_RS04425 (QDY30_04425) rsmE 903332..904063 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QDY30_RS04430 (QDY30_04430) endA 904143..904850 (-) 708 WP_098031165.1 deoxyribonuclease I -
  QDY30_RS04435 (QDY30_04435) yggI 904945..905442 (-) 498 WP_078167026.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35989.16 Da        Isoelectric Point: 5.5862

>NTDB_id=741109 QDY30_RS04405 WP_071777673.1 900110..901090(+) (pilT) [Escherichia coli strain ETEC6335]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLTENQRLRG
SAFAQRQGISLALRLLPSHYPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=741109 QDY30_RS04405 WP_071777673.1 900110..901090(+) (pilT) [Escherichia coli strain ETEC6335]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGACGGAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTACCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTTGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.438

98.16

0.377
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371