Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FPG73_RS00090 Genome accession   NZ_AP019794
Coordinates   17723..18808 (-) Length   361 a.a.
NCBI ID   WP_143584087.1    Uniprot ID   A0AAD1KS38
Organism   Thermus thermophilus strain AA2-29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 12723..23808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPG73_RS00075 (TthAA229_00120) - 14667..15299 (-) 633 WP_143584081.1 histidine phosphatase family protein -
  FPG73_RS00080 (TthAA229_00130) purM 15296..16297 (-) 1002 WP_143584083.1 phosphoribosylformylglycinamidine cyclo-ligase -
  FPG73_RS00085 (TthAA229_00140) gatB 16303..17712 (-) 1410 WP_143584085.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  FPG73_RS00090 (TthAA229_00150) pilT 17723..18808 (-) 1086 WP_143584087.1 type IV pilus twitching motility protein PilT Machinery gene
  FPG73_RS00095 (TthAA229_00160) pilF 18821..21487 (-) 2667 WP_143584089.1 type IV pilus assembly ATPase PilB Machinery gene
  FPG73_RS00100 (TthAA229_00170) - 21484..21969 (-) 486 WP_014628818.1 YqeG family HAD IIIA-type phosphatase -
  FPG73_RS00105 (TthAA229_00180) pgeF 22004..22732 (-) 729 WP_014628819.1 peptidoglycan editing factor PgeF -
  FPG73_RS00110 (TthAA229_00190) surE 22729..23484 (-) 756 WP_014628820.1 5'/3'-nucleotidase SurE -
  FPG73_RS00115 (TthAA229_00200) - 23543..23749 (-) 207 WP_143584091.1 cold-shock protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39934.27 Da        Isoelectric Point: 7.5112

>NTDB_id=74061 FPG73_RS00090 WP_143584087.1 17723..18808(-) (pilT) [Thermus thermophilus strain AA2-29]
MAKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSTVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLAAMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=74061 FPG73_RS00090 WP_143584087.1 17723..18808(-) (pilT) [Thermus thermophilus strain AA2-29]
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGGCGCTCGCGGTGGAGCGGGGGGCGAGCGACCTGGTGATCACCGTGGG
CCTGCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGGAAGGGGCGGTACCGGGTCAACGTCTTCCTGCAGCGGGGAAGCGTGGGGGGGGTTCTAAGGGTGGTGCCGAGCACCGT
GAAGAGCTTTGAGGAGCTCGGCCTTCCCAAGAACATCGCCGAGATCGCCATGAGCCCAAGGGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCTGGCAAGAGCACCACCCTGGCCGCCATGATCGACTACATCAACGAGCGCAAGGCCTGCCACATCGTC
ACCATCGAGGACCCCATAGAGTTCTTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAAACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTGTACAAGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.989

97.784

0.665
  pilT Pseudomonas aeruginosa PAK

54.36

95.291

0.518
  pilT Pseudomonas stutzeri DSM 10701

54.07

95.291

0.515
  pilT Acinetobacter baylyi ADP1

54.07

95.291

0.515
  pilT Acinetobacter baumannii strain A118

53.043

95.568

0.507
  pilT Acinetobacter nosocomialis M2

53.043

95.568

0.507
  pilT Acinetobacter baumannii D1279779

53.043

95.568

0.507
  pilT Neisseria meningitidis 8013

52.959

93.629

0.496
  pilT Neisseria gonorrhoeae MS11

52.226

93.352

0.488
  pilT Legionella pneumophila strain ERS1305867

50.872

95.291

0.485
  pilT Legionella pneumophila strain Lp02

50.872

95.291

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.976

91.136

0.474
  pilT Vibrio cholerae strain A1552

51.976

91.136

0.474
  pilU Pseudomonas stutzeri DSM 10701

46.939

95.014

0.446
  pilU Vibrio cholerae strain A1552

44.311

92.521

0.41
  pilU Acinetobacter baylyi ADP1

42.319

95.568

0.404

Multiple sequence alignment