Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5607_RS00155 Genome accession   NZ_AP019782
Coordinates   27853..28890 (+) Length   345 a.a.
NCBI ID   WP_054774927.1    Uniprot ID   A0A8D4VKH5
Organism   Methylogaea oryzae strain E10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 22853..33890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5607_RS00130 (MoryE10_00260) - 23231..25171 (-) 1941 WP_221047838.1 dynamin family protein -
  K5607_RS00135 (MoryE10_00270) - 25239..25544 (-) 306 WP_221047839.1 DUF167 family protein -
  K5607_RS00140 (MoryE10_00280) - 25544..26122 (-) 579 WP_221047840.1 YggT family protein -
  K5607_RS00145 (MoryE10_00290) proC 26122..26949 (-) 828 WP_221047841.1 pyrroline-5-carboxylate reductase -
  K5607_RS00150 (MoryE10_00300) - 26998..27723 (-) 726 WP_221047842.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5607_RS00155 (MoryE10_00310) pilT 27853..28890 (+) 1038 WP_054774927.1 type IV pilus twitching motility protein PilT Machinery gene
  K5607_RS00160 (MoryE10_00320) - 28938..30227 (-) 1290 WP_221047843.1 dihydroorotase -
  K5607_RS00165 (MoryE10_00330) - 30224..31201 (-) 978 WP_054774454.1 aspartate carbamoyltransferase catalytic subunit -
  K5607_RS00170 (MoryE10_00340) pyrR 31213..31725 (-) 513 WP_054774455.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  K5607_RS00175 (MoryE10_00350) ruvX 31722..32165 (-) 444 WP_221047844.1 Holliday junction resolvase RuvX -
  K5607_RS00180 (MoryE10_00360) - 32158..32721 (-) 564 WP_221047845.1 YqgE/AlgH family protein -
  K5607_RS00185 (MoryE10_00370) - 32766..33659 (-) 894 WP_221047846.1 TonB family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38362.96 Da        Isoelectric Point: 6.6657

>NTDB_id=73971 K5607_RS00155 WP_054774927.1 27853..28890(+) (pilT) [Methylogaea oryzae strain E10]
MDIAELLTFSVKNGASDLHLSAGMPPMIRVDGDMRKINVPPLDHKQTHALIYDIMNDKQRRDYEEFLETDFSFELPNVAR
FRVNAFNQDRGAAGVFRTIPSKILSLEQLNCPSSFAEFAAFPRGLVLVTGPTGSGKSTTLAAMINHINENDYSHILTVED
PIEFVHQSKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTINRI
IDVFPAAEKDMVRSMLSESLQAVISQTLLKKAEGGRVAAHEIMVGTPAIRNLIREDKVAQMYSAIQTGRKEGMQTLDQCL
QELVDKRLVTKNAAREKAANKSAFM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=73971 K5607_RS00155 WP_054774927.1 27853..28890(+) (pilT) [Methylogaea oryzae strain E10]
ATGGATATAGCCGAGCTGCTTACCTTTTCCGTCAAAAACGGTGCGTCCGACTTGCACCTGTCGGCGGGCATGCCGCCCAT
GATTCGCGTCGACGGCGACATGCGCAAGATCAACGTGCCGCCGCTGGACCATAAGCAGACCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGGCGCGATTACGAAGAGTTCCTGGAAACGGACTTCTCCTTCGAGCTGCCCAACGTGGCCCGC
TTCCGCGTCAACGCCTTCAACCAGGATCGCGGCGCCGCCGGGGTGTTCCGTACCATCCCGTCGAAAATCCTCAGCCTGGA
GCAACTCAACTGTCCGTCGTCCTTCGCCGAGTTCGCCGCTTTCCCGCGCGGCCTGGTGCTGGTGACGGGCCCCACCGGTT
CCGGCAAGTCCACCACCCTGGCGGCCATGATCAACCACATCAACGAAAACGATTATTCCCACATCCTCACGGTGGAGGAC
CCCATCGAATTCGTGCACCAAAGCAAGAAAAGCCTCATCAACCAGCGCGAGGTGCACCGCGACACCCTGGGTTTCAACGA
GGCGTTGCGCTCGGCCCTGCGCGAAGATCCGGACGTGATCCTGGTGGGCGAAATGCGCGACTTGGAAACCATCCGCCTGG
CCATGACCGCCGCCGAAACCGGCCACTTGGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCAACCGTATC
ATCGACGTGTTCCCGGCGGCGGAAAAAGACATGGTGCGCTCCATGCTGTCCGAATCCCTGCAAGCGGTGATTTCCCAGAC
CCTGCTGAAAAAAGCCGAGGGCGGCCGCGTGGCGGCGCACGAAATCATGGTGGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCGCCATCCAGACCGGCCGCAAGGAAGGCATGCAGACCTTGGATCAATGCCTG
CAAGAACTGGTGGACAAGCGGCTCGTCACCAAGAACGCCGCCAGGGAAAAAGCCGCCAACAAGTCGGCCTTTATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.261

100

0.783
  pilT Acinetobacter baumannii strain A118

78.261

100

0.783
  pilT Acinetobacter nosocomialis M2

78.261

100

0.783
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Legionella pneumophila strain Lp02

77.616

99.71

0.774
  pilT Legionella pneumophila strain ERS1305867

77.616

99.71

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.71

0.771
  pilT Acinetobacter baylyi ADP1

76.453

99.71

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.681

0.681
  pilT Vibrio cholerae strain A1552

69.733

97.681

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391
  pilU Pseudomonas stutzeri DSM 10701

41.358

93.913

0.388
  pilU Vibrio cholerae strain A1552

39.104

97.101

0.38

Multiple sequence alignment