Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5937_RS17125 Genome accession   NZ_CP107262
Coordinates   3454357..3455340 (+) Length   327 a.a.
NCBI ID   WP_159339851.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain 2022CK-00320     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3449357..3460340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5937_RS17090 (N5937_17090) - 3449738..3450064 (+) 327 WP_263950273.1 YggL family protein -
  N5937_RS17095 (N5937_17095) - 3450117..3450836 (+) 720 WP_103176932.1 DUF2884 domain-containing protein -
  N5937_RS17100 (N5937_17100) hemW 3451025..3452167 (-) 1143 WP_159339853.1 radical SAM family heme chaperone HemW -
  N5937_RS17105 (N5937_17105) - 3452160..3452753 (-) 594 WP_103176930.1 XTP/dITP diphosphatase -
  N5937_RS17110 (N5937_17110) yggU 3452757..3453053 (-) 297 WP_180209605.1 DUF167 family protein YggU -
  N5937_RS17115 (N5937_17115) - 3453050..3453616 (-) 567 WP_103176928.1 YggT family protein -
  N5937_RS17120 (N5937_17120) - 3453638..3454339 (-) 702 WP_263950274.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5937_RS17125 (N5937_17125) pilT 3454357..3455340 (+) 984 WP_159339851.1 type IV pilus twitching motility protein PilT Machinery gene
  N5937_RS17130 (N5937_17130) - 3455432..3456127 (+) 696 WP_103176925.1 transcriptional regulator -
  N5937_RS17135 (N5937_17135) ruvX 3456160..3456576 (-) 417 WP_180209603.1 Holliday junction resolvase RuvX -
  N5937_RS17140 (N5937_17140) - 3456576..3457136 (-) 561 WP_103176923.1 YqgE/AlgH family protein -
  N5937_RS17145 (N5937_17145) gshB 3457292..3458239 (-) 948 WP_263950275.1 glutathione synthase -
  N5937_RS17150 (N5937_17150) rsmE 3458259..3458990 (-) 732 WP_156655212.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N5937_RS17155 (N5937_17155) endA 3459081..3459788 (-) 708 WP_103176920.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 35825.13 Da        Isoelectric Point: 7.2149

>NTDB_id=738842 N5937_RS17125 WP_159339851.1 3454357..3455340(+) (pilT) [Leclercia adecarboxylata strain 2022CK-00320]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMEFAPFPGPEVAMLLKSWLSDEQQGAWCAQGQVDFAVTLASRQRLRA
SAFAHMQGCSLVLRLLPDACPQLRTLGAPRAIPELLANDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVE
FIHQSKRCLVQQREIGLHCLSFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDA
FPAQEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTWQLPGVIQTGQQAGMQNFEQSLAER
RAQGRLS

Nucleotide


Download         Length: 984 bp        

>NTDB_id=738842 N5937_RS17125 WP_159339851.1 3454357..3455340(+) (pilT) [Leclercia adecarboxylata strain 2022CK-00320]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGGCGGCGAGGGAAAATGGAATTTGCTCCCTTTCCGGGGCCGGAGGTCGCGATGCTGCTGAAAAGCTGGCTTAGCG
ACGAGCAGCAGGGGGCATGGTGCGCCCAAGGGCAGGTGGATTTTGCCGTTACGCTTGCGAGCAGGCAGCGGCTGCGGGCC
AGCGCCTTTGCGCATATGCAGGGGTGTTCTCTGGTGCTCCGCCTGCTTCCAGACGCGTGCCCGCAGTTGCGCACCCTGGG
AGCGCCGCGGGCGATACCCGAACTGCTGGCAAATGATAACGGTTTGATACTGGTGACCGGGGCGACAGGCAGCGGTAAAT
CCACCACCCTTGCGGCAATGGTCGATTTTCTCAATCACCATAGCGACGGGCATATCCTGACGCTCGAAGATCCGGTGGAA
TTTATCCATCAAAGCAAGCGTTGCCTGGTTCAGCAGCGGGAGATTGGCCTGCATTGCCTCTCCTTTGCCGACGCGCTGCG
GGCAGCCTTACGTGAAGATCCTGATGTGATCCTGCTGGGCGAACTGCGTGACAGCGAGACGATACGTCTTGCGCTTACCG
CCGCAGAGACCGGGCATCTGGTGCTGGCGACGCTCCACACCCGGGGCGCCGCCCAGGCAGTGGAGAGGCTGGTGGATGCC
TTCCCCGCGCAGGAAAAAGAGCCGGTACGGAAACAGCTGGCAGGCAGCCTGCGCGCCGTGCTGGCGCAAAAACTCGAAAG
GGATAATCAGCAGGGGCGCGTCGCGCTGTTCGAATTACTGGTTAATACCCCTGCGGCAGCCAATTTAATCCGCGAAGGAA
AAACCTGGCAACTGCCCGGCGTGATCCAGACCGGGCAGCAGGCTGGCATGCAAAACTTCGAGCAGAGCCTGGCTGAGCGC
AGGGCGCAGGGGCGATTGTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.495
  pilT Vibrio cholerae strain A1552

49.541

100

0.495
  pilT Neisseria meningitidis 8013

49.085

100

0.492
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.489
  pilT Legionella pneumophila strain ERS1305867

48.447

98.471

0.477
  pilT Legionella pneumophila strain Lp02

48.447

98.471

0.477
  pilT Pseudomonas stutzeri DSM 10701

46.97

100

0.474
  pilT Acinetobacter baumannii strain A118

46.364

100

0.468
  pilT Acinetobacter baumannii D1279779

46.364

100

0.468
  pilT Pseudomonas aeruginosa PAK

46.364

100

0.468
  pilT Acinetobacter nosocomialis M2

46.061

100

0.465
  pilT Acinetobacter baylyi ADP1

46.483

100

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.471

0.428
  pilU Vibrio cholerae strain A1552

39.697

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

38.369

100

0.388
  pilU Acinetobacter baylyi ADP1

37.231

99.388

0.37