Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCT39_RS15170 Genome accession   NZ_CP107007
Coordinates   3292747..3293790 (-) Length   347 a.a.
NCBI ID   WP_263585280.1    Uniprot ID   A0AA96EC85
Organism   Halomonas sp. GD1P12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3287747..3298790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCT39_RS15160 (OCT39_15160) tyrS 3290265..3291461 (-) 1197 WP_263585278.1 tyrosine--tRNA ligase -
  OCT39_RS15165 (OCT39_15165) - 3291585..3292739 (+) 1155 WP_263585279.1 anhydro-N-acetylmuramic acid kinase -
  OCT39_RS15170 (OCT39_15170) pilT 3292747..3293790 (-) 1044 WP_263585280.1 type IV pilus twitching motility protein PilT Machinery gene
  OCT39_RS15175 (OCT39_15175) - 3293823..3294527 (+) 705 WP_263585281.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCT39_RS15180 (OCT39_15180) proC 3294593..3295417 (+) 825 WP_263585282.1 pyrroline-5-carboxylate reductase -
  OCT39_RS15185 (OCT39_15185) - 3295615..3296208 (+) 594 WP_263585283.1 YggT family protein -
  OCT39_RS15190 (OCT39_15190) metX 3296246..3297442 (+) 1197 WP_263585284.1 homoserine O-succinyltransferase MetX -
  OCT39_RS15195 (OCT39_15195) metW 3297442..3298035 (+) 594 WP_263587354.1 methionine biosynthesis protein MetW -
  OCT39_RS15200 (OCT39_15200) sfsA 3298032..3298763 (-) 732 WP_263585285.1 DNA/RNA nuclease SfsA -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38087.63 Da        Isoelectric Point: 6.0873

>NTDB_id=737306 OCT39_RS15170 WP_263585280.1 3292747..3293790(-) (pilT) [Halomonas sp. GD1P12]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIHRLNVPAMSHEQVLSLVHAIMNDRQRQEYDEHLEIDFSYALAEVAR
FRVNAFTQGRGAGAVFRTIPSQVLSMKALGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAALIDYINEYRYEHILTIED
PVEFVHESKSCLVNQREVHRDTHSFANALKSALREDPDVIMVGELRDLETIRLALTAAETGHLVFATLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVVAQTLLKREGGGRVAAHEILIATSAVRNLIREDKVAQIYSAMQTGVNLGMQTLNASL
LKLVKEGAVSFEEAQTRAQGALTPPRD

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=737306 OCT39_RS15170 WP_263585280.1 3292747..3293790(-) (pilT) [Halomonas sp. GD1P12]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAACAGAATGCCTCCGACCTGCATCTGAGCGCCGGTTTGCCGCCCAT
GATTCGCGTCGATGGCGATATTCATCGGCTCAACGTCCCCGCCATGAGCCACGAACAGGTGCTGTCGCTGGTTCACGCCA
TCATGAACGACCGTCAACGCCAGGAGTATGACGAGCATCTGGAGATCGACTTCTCCTACGCCTTGGCGGAGGTCGCACGC
TTTCGCGTCAACGCCTTCACCCAGGGGCGTGGCGCCGGGGCGGTGTTTCGCACCATCCCAAGCCAGGTGCTGTCGATGAA
AGCGCTGGGCCTGGGAGAGGTATTCGAGCGCCTGGCAATGCTGCCGCGTGGGCTGGTGCTGGTCACCGGCCCGACCGGGT
CGGGCAAGAGTACGACCCTGGCCGCGCTGATCGACTACATCAACGAATACCGCTATGAGCACATTCTCACCATCGAAGAC
CCGGTGGAATTCGTGCACGAAAGCAAGAGCTGTCTGGTCAATCAACGCGAAGTGCACCGCGATACCCACAGCTTCGCCAA
CGCCTTGAAAAGCGCGCTGCGTGAGGATCCGGACGTGATCATGGTGGGGGAGCTTCGCGATCTCGAAACCATTCGGCTGG
CGCTGACCGCGGCGGAAACCGGGCACCTGGTCTTCGCCACGCTGCACACCACCTCGGCGGCGAAGACCATTGATCGCATC
ATCGACGTTTTCCCCGGCGAGGAGAAAGCCATGGTGCGCTCGATGCTATCGGAGTCGCTGCAGGCGGTCGTCGCCCAGAC
GCTGTTAAAGCGCGAAGGCGGCGGGCGCGTGGCGGCCCACGAAATCCTGATCGCCACCTCGGCGGTGCGCAACCTCATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCGATGCAGACCGGCGTCAATCTCGGCATGCAGACCCTCAACGCTTCGCTA
TTAAAGCTGGTCAAGGAGGGCGCGGTCAGCTTCGAGGAGGCTCAGACCCGCGCCCAGGGCGCGCTTACTCCGCCCCGCGA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

74.041

97.695

0.723
  pilT Pseudomonas stutzeri DSM 10701

73.451

97.695

0.718
  pilT Acinetobacter nosocomialis M2

72.566

97.695

0.709
  pilT Acinetobacter baumannii D1279779

72.566

97.695

0.709
  pilT Acinetobacter baumannii strain A118

72.566

97.695

0.709
  pilT Acinetobacter baylyi ADP1

70.501

97.695

0.689
  pilT Legionella pneumophila strain Lp02

70.606

95.101

0.671
  pilT Legionella pneumophila strain ERS1305867

70.606

95.101

0.671
  pilT Neisseria gonorrhoeae MS11

66.374

98.559

0.654
  pilT Neisseria meningitidis 8013

66.374

98.559

0.654
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.667

97.695

0.651
  pilT Vibrio cholerae strain A1552

66.667

97.695

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.622

97.695

0.504
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Vibrio cholerae strain A1552

41.52

98.559

0.409
  pilU Acinetobacter baylyi ADP1

40.476

96.83

0.392