Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P9060_RS04350 Genome accession   NZ_CP121356
Coordinates   882216..883196 (+) Length   326 a.a.
NCBI ID   WP_050487204.1    Uniprot ID   -
Organism   Escherichia coli strain rl0044     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 877216..888196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P9060_RS04320 (P9060_04320) yggM 877731..878738 (+) 1008 WP_000745250.1 DUF1202 family protein -
  P9060_RS04325 (P9060_04325) hemW 878893..880029 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  P9060_RS04330 (P9060_04330) rdgB 880022..880615 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  P9060_RS04335 (P9060_04335) yggU 880623..880913 (-) 291 WP_000994920.1 DUF167 family protein YggU -
  P9060_RS04340 (P9060_04340) yggT 880910..881476 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  P9060_RS04345 (P9060_04345) yggS 881494..882198 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  P9060_RS04350 (P9060_04350) pilT 882216..883196 (+) 981 WP_050487204.1 type IV pilus twitching motility protein PilT Machinery gene
  P9060_RS04355 (P9060_04355) ruvX 883380..883796 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  P9060_RS04360 (P9060_04360) yqgE 883796..884359 (-) 564 WP_001460055.1 YqgE/AlgH family protein -
  P9060_RS04365 (P9060_04365) gshB 884468..885418 (-) 951 WP_000593273.1 glutathione synthase -
  P9060_RS04370 (P9060_04370) rsmE 885431..886162 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  P9060_RS04375 (P9060_04375) endA 886242..886949 (-) 708 WP_000286500.1 deoxyribonuclease I -
  P9060_RS04380 (P9060_04380) yggI 887044..887541 (-) 498 WP_001460054.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36009.17 Da        Isoelectric Point: 5.7404

>NTDB_id=736983 P9060_RS04350 WP_050487204.1 882216..883196(+) (pilT) [Escherichia coli strain rl0044]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATESGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQSVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=736983 P9060_RS04350 WP_050487204.1 882216..883196(+) (pilT) [Escherichia coli strain rl0044]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGAGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGTCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.597

97.546

0.445
  pilT Legionella pneumophila strain Lp02

45.597

97.546

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.446

100

0.371