Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCT50_RS18135 Genome accession   NZ_CP106959
Coordinates   3886659..3887636 (-) Length   325 a.a.
NCBI ID   WP_289896954.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain 17YN198     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3881659..3892636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCT50_RS18105 (OCT50_18100) endA 3882245..3882952 (+) 708 WP_289896953.1 deoxyribonuclease I -
  OCT50_RS18110 (OCT50_18105) rsmE 3883064..3883795 (+) 732 WP_032613955.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCT50_RS18115 (OCT50_18110) gshB 3883815..3884762 (+) 948 WP_059307231.1 glutathione synthase -
  OCT50_RS18120 (OCT50_18115) - 3884854..3885414 (+) 561 WP_032613928.1 YqgE/AlgH family protein -
  OCT50_RS18125 (OCT50_18120) ruvX 3885414..3885830 (+) 417 WP_130588229.1 Holliday junction resolvase RuvX -
  OCT50_RS18130 (OCT50_18125) - 3885870..3886559 (-) 690 WP_032613957.1 hypothetical protein -
  OCT50_RS18135 (OCT50_18130) pilT 3886659..3887636 (-) 978 WP_289896954.1 type IV pilus twitching motility protein PilT Machinery gene
  OCT50_RS18140 (OCT50_18135) - 3887654..3888355 (+) 702 WP_032613935.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCT50_RS18145 (OCT50_18140) - 3888377..3888943 (+) 567 WP_032613937.1 YggT family protein -
  OCT50_RS18150 (OCT50_18145) yggU 3888940..3889236 (+) 297 WP_032613939.1 DUF167 family protein YggU -
  OCT50_RS18155 (OCT50_18150) - 3889240..3889833 (+) 594 WP_059307228.1 XTP/dITP diphosphatase -
  OCT50_RS18160 (OCT50_18155) hemW 3889826..3890968 (+) 1143 WP_059307227.1 radical SAM family heme chaperone HemW -
  OCT50_RS18165 (OCT50_18160) - 3891032..3891394 (+) 363 Protein_3551 endonuclease domain-containing protein -
  OCT50_RS18170 (OCT50_18165) - 3891624..3892343 (-) 720 WP_077227222.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35661.89 Da        Isoelectric Point: 7.2814

>NTDB_id=736842 OCT50_RS18135 WP_289896954.1 3886659..3887636(-) (pilT) [Leclercia adecarboxylata strain 17YN198]
MNMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMELAPFPGPDVVMLLKSWLSDEQQGAWCAQGQVDFAVTLKSHRLRAS
AFAHTQGCSLALRLLPEQCPQLHSLGTPRAIPELLTRDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNQQGRVALFELLINTSAAANLIREGKTYQLPGVIQTGLQAGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=736842 OCT50_RS18135 WP_289896954.1 3886659..3887636(-) (pilT) [Leclercia adecarboxylata strain 17YN198]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACGAGGAAAGATGGAATTGGCGCCTTTTCCGGGGCCGGATGTGGTGATGCTGCTGAAAAGCTGGCTCAGCG
ATGAGCAGCAGGGGGCATGGTGTGCTCAGGGGCAGGTAGATTTTGCCGTCACACTGAAGAGCCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCAGAACAGTGCCCGCAATTGCATAGTCTGGGTAC
GCCGCGGGCGATCCCCGAGCTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGTGCCACCGGCAGCGGCAAGTCCA
CCACGCTTGCGGCAATGGTCGACTTTCTTAATCACCACAGCGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGCAACGCCTTTGCCGATGCGCTGCGGGC
GGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAACTGCGCGATAGCGAAACGATCCGCCTTGCGCTTACCGCCG
CAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACCCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAGCTGGCCGGCAGTTTGCAGGCGGTGCTGGCACAAAAACTCGAAAGGGA
CAACCAGCAGGGGCGCGTGGCGCTGTTTGAGTTACTCATTAACACCTCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGCGTGATCCAGACCGGGCTGCAGGCAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.486
  pilT Vibrio cholerae strain A1552

48.318

100

0.486
  pilT Legionella pneumophila strain Lp02

47.826

99.077

0.474
  pilT Legionella pneumophila strain ERS1305867

47.826

99.077

0.474
  pilT Neisseria meningitidis 8013

46.646

100

0.471
  pilT Pseudomonas stutzeri DSM 10701

47.95

97.538

0.468
  pilT Acinetobacter baylyi ADP1

46.483

100

0.468
  pilT Pseudomonas aeruginosa PAK

47.319

97.538

0.462
  pilT Acinetobacter nosocomialis M2

47.022

98.154

0.462
  pilT Acinetobacter baumannii strain A118

47.022

98.154

0.462
  pilT Acinetobacter baumannii D1279779

47.022

98.154

0.462
  pilT Neisseria gonorrhoeae MS11

46.875

98.462

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.994

96.615

0.415
  pilU Vibrio cholerae strain A1552

39.937

97.846

0.391
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.382
  pilU Acinetobacter baylyi ADP1

36.42

99.692

0.363