Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N7922_RS04090 Genome accession   NZ_CP106754
Coordinates   857347..858327 (+) Length   326 a.a.
NCBI ID   WP_263099369.1    Uniprot ID   -
Organism   Kosakonia sp. ML.JS2a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 852347..863327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7922_RS04055 (N7922_04055) - 852722..853441 (+) 720 WP_263099362.1 DUF2884 domain-containing protein -
  N7922_RS04060 (N7922_04060) - 853588..853947 (-) 360 WP_263099363.1 endonuclease domain-containing protein -
  N7922_RS04065 (N7922_04065) hemW 854022..855158 (-) 1137 WP_263099364.1 radical SAM family heme chaperone HemW -
  N7922_RS04070 (N7922_04070) - 855151..855744 (-) 594 WP_263099365.1 XTP/dITP diphosphatase -
  N7922_RS04075 (N7922_04075) yggU 855753..856043 (-) 291 WP_263099366.1 DUF167 family protein YggU -
  N7922_RS04080 (N7922_04080) - 856040..856606 (-) 567 WP_263099367.1 YggT family protein -
  N7922_RS04085 (N7922_04085) - 856625..857329 (-) 705 WP_263099368.1 YggS family pyridoxal phosphate-dependent enzyme -
  N7922_RS04090 (N7922_04090) pilT 857347..858327 (+) 981 WP_263099369.1 type IV pilus twitching motility protein PilT Machinery gene
  N7922_RS04095 (N7922_04095) - 858324..860585 (-) 2262 WP_263099370.1 FAD-dependent oxidoreductase -
  N7922_RS04100 (N7922_04100) - 860653..861345 (+) 693 WP_263099371.1 IclR family transcriptional regulator -
  N7922_RS04105 (N7922_04105) ruvX 861508..861924 (-) 417 WP_263099372.1 Holliday junction resolvase RuvX -
  N7922_RS04110 (N7922_04110) - 861924..862487 (-) 564 WP_263099373.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35591.69 Da        Isoelectric Point: 6.7713

>NTDB_id=734490 N7922_RS04090 WP_263099369.1 857347..858327(+) (pilT) [Kosakonia sp. ML.JS2a]
MDIEKIVELSVKHNVSDLHLCSGRSPRWRQQGRLEPAPFPCPDIAALMNRHLSEMLQSQWREQGQVDFALTTASGLRLRA
GAFARHDGISLALRLLPQRCPQLSELDAPQALTGLLAQEHGLILVTGATGSGKSTTLSAMVNHLNQRLDGHIVTLEDPIE
FVHTSERCLIQQREVGLHCPSFASGLRAALREDPDVILLGELRDSDTIWLALTAAETGHLVLATLHTRGAAQAVERLVDA
FPAQEKDPVRSQLAGSLLAVLAQKLEADKQEGRVALFELLVNTPASANLIREGKTHQLPGVMQTGQQAGMLTFAQSRQQR
EQAGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=734490 N7922_RS04090 WP_263099369.1 857347..858327(+) (pilT) [Kosakonia sp. ML.JS2a]
ATGGATATAGAAAAAATAGTGGAGCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGTGGGCGTTCTCCACG
CTGGCGGCAACAAGGGCGGCTGGAGCCCGCGCCTTTTCCCTGTCCGGACATCGCTGCACTCATGAACAGGCATCTTAGCG
AAATGCTGCAAAGCCAGTGGCGGGAGCAGGGGCAGGTGGATTTTGCGCTCACCACTGCCAGCGGTCTGCGTTTGCGCGCC
GGGGCGTTTGCCCGTCATGACGGTATTTCACTGGCGCTGCGGTTATTACCGCAACGCTGCCCGCAGCTCAGCGAACTTGA
TGCGCCACAGGCGCTTACCGGGTTGCTCGCGCAGGAACACGGTTTGATTCTGGTGACCGGCGCTACAGGCAGCGGTAAAT
CGACAACGCTTTCGGCAATGGTTAATCACCTTAATCAGCGCCTGGACGGGCATATAGTGACGCTTGAAGATCCGATTGAG
TTTGTGCACACCAGCGAACGTTGCTTAATCCAGCAGCGGGAGGTCGGTCTGCACTGCCCATCATTTGCCAGCGGACTGCG
TGCGGCATTACGTGAGGATCCGGATGTGATCCTGCTTGGCGAGTTGCGCGACAGTGATACGATCTGGCTGGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGCGCAGCGCAGGCGGTGGAACGGCTGGTGGATGCT
TTCCCGGCGCAGGAAAAAGACCCTGTGCGCAGCCAGCTTGCCGGAAGTTTACTGGCGGTGCTGGCGCAAAAACTGGAAGC
CGATAAACAAGAAGGGCGGGTCGCGCTGTTTGAACTGCTGGTGAATACCCCGGCATCGGCGAACCTGATCCGCGAAGGCA
AGACGCATCAACTGCCCGGTGTGATGCAAACCGGGCAGCAGGCGGGGATGCTGACATTTGCGCAAAGCCGTCAGCAACGC
GAACAGGCGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.689

98.773

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.689

98.773

0.491
  pilT Neisseria meningitidis 8013

46.894

98.773

0.463
  pilT Neisseria gonorrhoeae MS11

46.584

98.773

0.46
  pilT Acinetobacter baylyi ADP1

44.72

98.773

0.442
  pilT Pseudomonas stutzeri DSM 10701

44.937

96.933

0.436
  pilT Acinetobacter nosocomialis M2

44.201

97.853

0.433
  pilT Acinetobacter baumannii strain A118

44.201

97.853

0.433
  pilT Acinetobacter baumannii D1279779

44.201

97.853

0.433
  pilT Pseudomonas aeruginosa PAK

44.62

96.933

0.433
  pilT Legionella pneumophila strain ERS1305867

44.025

97.546

0.429
  pilT Legionella pneumophila strain Lp02

44.025

97.546

0.429
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.082

97.546

0.42
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Pseudomonas stutzeri DSM 10701

37.812

98.16

0.371
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368