Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N8O08_RS03370 Genome accession   NZ_CP104976
Coordinates   699986..700966 (+) Length   326 a.a.
NCBI ID   WP_223561514.1    Uniprot ID   -
Organism   Enterobacter sp. 155105     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 694986..705966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8O08_RS03330 (N8O08_03330) - 695370..695696 (+) 327 WP_014885198.1 YggL family protein -
  N8O08_RS03335 (N8O08_03335) - 695754..696470 (+) 717 WP_223561518.1 DUF2884 domain-containing protein -
  N8O08_RS03340 (N8O08_03340) - 696492..696629 (-) 138 Protein_647 DUF559 domain-containing protein -
  N8O08_RS03345 (N8O08_03345) hemW 696654..697796 (-) 1143 WP_223561517.1 radical SAM family heme chaperone HemW -
  N8O08_RS03350 (N8O08_03350) - 697789..698382 (-) 594 WP_223561516.1 XTP/dITP diphosphatase -
  N8O08_RS03355 (N8O08_03355) yggU 698386..698682 (-) 297 WP_023309131.1 DUF167 family protein YggU -
  N8O08_RS03360 (N8O08_03360) - 698679..699245 (-) 567 WP_010435467.1 YggT family protein -
  N8O08_RS03365 (N8O08_03365) - 699267..699968 (-) 702 WP_223561515.1 YggS family pyridoxal phosphate-dependent enzyme -
  N8O08_RS03370 (N8O08_03370) pilT 699986..700966 (+) 981 WP_223561514.1 type IV pilus twitching motility protein PilT Machinery gene
  N8O08_RS03375 (N8O08_03375) ruvX 700977..701393 (-) 417 WP_223561513.1 Holliday junction resolvase RuvX -
  N8O08_RS03380 (N8O08_03380) - 701393..701953 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  N8O08_RS03385 (N8O08_03385) gshB 702041..702988 (-) 948 WP_223561512.1 glutathione synthase -
  N8O08_RS03390 (N8O08_03390) rsmE 703008..703739 (-) 732 WP_223561511.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N8O08_RS03395 (N8O08_03395) endA 703791..704498 (-) 708 WP_223561510.1 deoxyribonuclease I -
  N8O08_RS03400 (N8O08_03400) - 704593..705090 (-) 498 WP_223561509.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35492.44 Da        Isoelectric Point: 6.4988

>NTDB_id=733638 N8O08_RS03370 WP_223561514.1 699986..700966(+) (pilT) [Enterobacter sp. 155105]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWANGQVDFAATVTGGQRLRG
SAFKQMSGISVTLRLLPRTCPQLTSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=733638 N8O08_RS03370 WP_223561514.1 699986..700966(+) (pilT) [Enterobacter sp. 155105]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAGCCTGCTCCCTTTCCGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCGAACGGGCAGGTGGACTTTGCCGCGACGGTGACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGCGGTATCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGCCCGCAGCTCACTTCGCTGGG
CGCGCCGCGGGCAATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTGACCGGGGCAACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTTAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGTGAACGTTGCCTGATCCAGCAGAGGGAGATAGGCCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
TAGTGCCTTACGCCAGGATCCGGACGTTATTTTACTGGGAGAGTTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACTG
CAGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGTCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCTGGCAGCCTGAGGGCGGTGCTGGCGCAAAGGCTGCTTCC
CGATCTACAGGGAGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACCCCAGCGGCGGCGAATCTTATTCGTGAAGGGA
AAACGTGGCAACTTCCCGGCATCATCCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCAGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.405
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365