Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P5711_RS04260 Genome accession   NZ_CP120568
Coordinates   868882..869862 (+) Length   326 a.a.
NCBI ID   WP_072056733.1    Uniprot ID   -
Organism   Escherichia coli strain 582     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 863882..874862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5711_RS04225 (P5711_04225) - 864184..864689 (-) 506 Protein_829 TRAP transporter large permease subunit -
  P5711_RS04230 (P5711_04230) - 864732..865429 (+) 698 WP_223429336.1 IS1 family transposase -
  P5711_RS04235 (P5711_04235) hemW 865559..866695 (-) 1137 WP_001521257.1 radical SAM family heme chaperone HemW -
  P5711_RS04240 (P5711_04240) rdgB 866688..867281 (-) 594 WP_001174754.1 XTP/dITP diphosphatase -
  P5711_RS04245 (P5711_04245) yggU 867289..867579 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  P5711_RS04250 (P5711_04250) yggT 867576..868142 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  P5711_RS04255 (P5711_04255) yggS 868160..868864 (-) 705 WP_046952504.1 pyridoxal phosphate homeostasis protein -
  P5711_RS04260 (P5711_04260) pilT 868882..869862 (+) 981 WP_072056733.1 type IV pilus twitching motility protein PilT Machinery gene
  P5711_RS04265 (P5711_04265) ruvX 869977..870393 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  P5711_RS04270 (P5711_04270) yqgE 870393..870956 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  P5711_RS04275 (P5711_04275) gshB 871065..872015 (-) 951 WP_000593273.1 glutathione synthase -
  P5711_RS04280 (P5711_04280) rsmE 872028..872759 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  P5711_RS04285 (P5711_04285) endA 872839..873546 (-) 708 WP_000286497.1 deoxyribonuclease I -
  P5711_RS04290 (P5711_04290) yggI 873641..874138 (-) 498 WP_001296359.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35986.17 Da        Isoelectric Point: 5.7980

>NTDB_id=732556 P5711_RS04260 WP_072056733.1 868882..869862(+) (pilT) [Escherichia coli strain 582]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAIMLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAALVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=732556 P5711_RS04260 WP_072056733.1 868882..869862(+) (pilT) [Escherichia coli strain 582]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATAATGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCGCCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGTAAAT
CCACCACGCTGGCGGCATTGGTTGGCTATCTTAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGAGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACGCCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365