Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5056_RS17400 Genome accession   NZ_CP104757
Coordinates   3899736..3900752 (-) Length   338 a.a.
NCBI ID   WP_181829234.1    Uniprot ID   -
Organism   Pectobacterium aroidearum strain QJ021     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3894736..3905752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5056_RS17385 (N5056_17385) nsrR 3895100..3895525 (-) 426 WP_015841587.1 nitric oxide-sensing transcriptional repressor NsrR -
  N5056_RS17390 (N5056_17390) - 3895842..3897413 (+) 1572 WP_181829232.1 methyl-accepting chemotaxis protein -
  N5056_RS17395 (N5056_17395) - 3897941..3899767 (+) 1827 WP_181829233.1 diguanylate cyclase -
  N5056_RS17400 (N5056_17400) pilT 3899736..3900752 (-) 1017 WP_181829234.1 type IV pilus twitching motility protein PilT Machinery gene
  N5056_RS17405 (N5056_17405) - 3900774..3901487 (+) 714 WP_181829235.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5056_RS17410 (N5056_17410) proC 3901578..3902399 (+) 822 WP_015841592.1 pyrroline-5-carboxylate reductase -
  N5056_RS17415 (N5056_17415) - 3902448..3903002 (+) 555 WP_010295003.1 YggT family protein -
  N5056_RS17420 (N5056_17420) yggU 3902999..3903289 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  N5056_RS17425 (N5056_17425) - 3903307..3903900 (+) 594 WP_181829236.1 XTP/dITP diphosphatase -
  N5056_RS17430 (N5056_17430) hemW 3903893..3905035 (+) 1143 WP_181829237.1 radical SAM family heme chaperone HemW -
  N5056_RS17435 (N5056_17435) - 3905264..3905524 (+) 261 WP_181829238.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36889.10 Da        Isoelectric Point: 6.7684

>NTDB_id=732054 N5056_RS17400 WP_181829234.1 3899736..3900752(-) (pilT) [Pectobacterium aroidearum strain QJ021]
MELDEWIARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSEQVAQWCDAWLEPAQREQLRQFGQIDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLDGLHAPPVLSTLLEKTNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRATLREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRTASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPSAHGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGTAAGMQTFEQS
YQQRCRDGLISHNCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=732054 N5056_RS17400 WP_181829234.1 3899736..3900752(-) (pilT) [Pectobacterium aroidearum strain QJ021]
ATGGAATTGGATGAGTGGATAGCGCGTAGTGTAAAACATAATGCCTCAGATCTGCACCTTTGTAGCGGCCACCCGCCGGT
GTTACGTGTCGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCCGCTCAGAGAGAACAACTGCGGCAGTTTGGTCAGATAGACGGTGCGTTAATGCTGCCTGATGGGCAG
CGTCTGCGGGTAAATGTGTTTCGCCAGCGAGAAGGGCTGTCCGCGGCGCTGCGTATTATCCCGTCCGTTCAGCCGTCATT
GGACGGGCTACACGCGCCACCCGTTCTGTCGACGCTGTTGGAAAAAACGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCAATGCTCGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGGCGCTGCCTGATTCAACAGCGAGAGATCGGTGCGCACAGCGCGTCTTTTGC
ACAGGCGCTACGGGCCACCTTGCGGGAAGATCCCGATGTGATTCTGCTGGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACAGCGGCGGAAACCGGCCATCTGGTGCTATCCACGCTGCATACGCGCACTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGTCAGCTAGCCACCTGCTTGCAGGCCGTGGTGACGCA
AAGGTTGTTGCCTTCTGCACACGGCGGTCGTATTGCGCTTTATGAAGTGCTGACGGCGACAGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCATCAGCTTCCCGGCCTGATTCAAACGGGAACGGCTGCGGGCATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAATTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.405

99.408

0.491
  pilT Vibrio cholerae strain A1552

50.303

97.633

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

97.633

0.491
  pilT Neisseria gonorrhoeae MS11

49.107

99.408

0.488
  pilT Legionella pneumophila strain ERS1305867

48.358

99.112

0.479
  pilT Legionella pneumophila strain Lp02

48.358

99.112

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.358

99.112

0.479
  pilT Pseudomonas aeruginosa PAK

48.06

99.112

0.476
  pilT Acinetobacter baylyi ADP1

47.633

100

0.476
  pilT Acinetobacter nosocomialis M2

47.164

99.112

0.467
  pilT Acinetobacter baumannii D1279779

47.164

99.112

0.467
  pilT Acinetobacter baumannii strain A118

47.164

99.112

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.95

99.704

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.653

99.704

0.405
  pilU Vibrio cholerae strain A1552

39.169

99.704

0.391
  pilU Acinetobacter baylyi ADP1

39.13

95.266

0.373