Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NAL19_RS17320 Genome accession   NZ_CP104733
Coordinates   3868292..3869308 (-) Length   338 a.a.
NCBI ID   WP_263057987.1    Uniprot ID   -
Organism   Pectobacterium sp. F1-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3863292..3874308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAL19_RS17300 (NAL19_3719) nsrR 3863680..3864105 (-) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  NAL19_RS17305 (NAL19_3720) - 3864400..3865971 (+) 1572 WP_225085674.1 methyl-accepting chemotaxis protein -
  NAL19_RS17310 - 3866499..3866820 (+) 322 Protein_3389 PAS domain S-box protein -
  NAL19_RS17315 (NAL19_3721) - 3866887..3868323 (+) 1437 WP_263059742.1 diguanylate cyclase -
  NAL19_RS17320 (NAL19_3722) pilT 3868292..3869308 (-) 1017 WP_263057987.1 type IV pilus twitching motility protein PilT Machinery gene
  NAL19_RS17325 (NAL19_3723) - 3869329..3870057 (+) 729 WP_225085677.1 YggS family pyridoxal phosphate-dependent enzyme -
  NAL19_RS17330 (NAL19_3724) proC 3870148..3870969 (+) 822 WP_263057988.1 pyrroline-5-carboxylate reductase -
  NAL19_RS17335 (NAL19_3725) - 3871018..3871572 (+) 555 WP_010295003.1 YggT family protein -
  NAL19_RS17340 (NAL19_3726) yggU 3871569..3871859 (+) 291 WP_225085679.1 DUF167 family protein YggU -
  NAL19_RS17345 (NAL19_3727) - 3871891..3872484 (+) 594 WP_263057989.1 XTP/dITP diphosphatase -
  NAL19_RS17350 (NAL19_3728) hemW 3872477..3873619 (+) 1143 WP_263057990.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36837.19 Da        Isoelectric Point: 6.8191

>NTDB_id=731664 NAL19_RS17320 WP_263057987.1 3868292..3869308(-) (pilT) [Pectobacterium sp. F1-1]
MELDEWMVRSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCNAWLEPAQREQLRQFGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLDGLQAPPVLSMLLEKPNGLILITGATGSGKSTTLAAMIGALNDSSDRHVITLE
DPIEFIHVSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTDTIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGTAAGMQTFEQS
YQQRCRDGLISHSCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=731664 NAL19_RS17320 WP_263057987.1 3868292..3869308(-) (pilT) [Pectobacterium sp. F1-1]
ATGGAATTGGATGAGTGGATGGTGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGCCACCCTCCGGT
GCTACGAGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAGCAGGTGGCGCAATGGTGCAATG
CCTGGCTCGAACCCGCGCAGCGAGAGCAACTGCGGCAGTTTGGTCAGGTGGACGGCGCGTTAATGCTGCCCGATGGGCAA
CGTCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTATTATTCCGTCGGCTCAGCCTTCATT
GGACGGGCTACAGGCGCCACCCGTTCTGTCGATGCTGCTGGAGAAACCGAACGGACTGATCCTGATTACTGGTGCGACGG
GGAGTGGCAAGTCCACGACGCTGGCAGCCATGATCGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACACTGGAG
GATCCGATCGAGTTTATCCATGTCAGCCGGCGTTGCCTGATTCAACAGCGAGAGATCGGTGCACACAGCGCGTCTTTTGC
GCAGGCGTTACGGGCGGCATTGCGGGAAGATCCCGATGTGATTCTGCTGGGGGAACTTCGTGATACAGATACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTATCTACGCTGCATACACGCAGCGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAAAAAGCCTATGTTCGCAGCCAGCTCGCCACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGTTGCCTGCAGCGCAAGGCGGCCGCATTGCGCTTTATGAAGTGCTGACGGCGACTGCGGCGGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCATCAGCTTCCCGGCCTGATTCAAACGGGAACGGCGGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

99.408

0.497
  pilT Vibrio cholerae strain A1552

50.606

97.633

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.606

97.633

0.494
  pilT Neisseria gonorrhoeae MS11

49.702

99.408

0.494
  pilT Legionella pneumophila strain ERS1305867

48.657

99.112

0.482
  pilT Legionella pneumophila strain Lp02

48.657

99.112

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.358

99.112

0.479
  pilT Pseudomonas aeruginosa PAK

48.06

99.112

0.476
  pilT Acinetobacter baylyi ADP1

47.633

100

0.476
  pilT Acinetobacter nosocomialis M2

47.164

99.112

0.467
  pilT Acinetobacter baumannii D1279779

47.164

99.112

0.467
  pilT Acinetobacter baumannii strain A118

47.164

99.112

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.84

99.704

0.417
  pilU Pseudomonas stutzeri DSM 10701

40.653

99.704

0.405
  pilU Vibrio cholerae strain A1552

38.872

99.704

0.388
  pilU Acinetobacter baylyi ADP1

37.574

100

0.376