Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N4534_RS03570 Genome accession   NZ_CP104677
Coordinates   738965..739945 (+) Length   326 a.a.
NCBI ID   WP_001055658.1    Uniprot ID   A0A0R9N9F2
Organism   Salmonella enterica strain PNUSAS036471     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733965..744945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4534_RS03540 (N4534_03540) - 734542..735549 (+) 1008 WP_000252198.1 DUF1202 domain-containing protein -
  N4534_RS03545 (N4534_03545) hemW 735641..736777 (-) 1137 WP_001096525.1 radical SAM family heme chaperone HemW -
  N4534_RS03550 (N4534_03550) - 736770..737363 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  N4534_RS03555 (N4534_03555) yggU 737371..737661 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  N4534_RS03560 (N4534_03560) - 737658..738224 (-) 567 WP_001094848.1 YggT family protein -
  N4534_RS03565 (N4534_03565) - 738243..738947 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4534_RS03570 (N4534_03570) pilT 738965..739945 (+) 981 WP_001055658.1 type IV pilus twitching motility protein PilT Machinery gene
  N4534_RS03575 (N4534_03575) - 740080..740766 (+) 687 WP_000098321.1 IclR family transcriptional regulator -
  N4534_RS03580 (N4534_03580) ruvX 740813..741229 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  N4534_RS03585 (N4534_03585) - 741229..741792 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  N4534_RS03590 (N4534_03590) gshB 742008..742955 (-) 948 WP_000593248.1 glutathione synthase -
  N4534_RS03595 (N4534_03595) rsmE 742975..743706 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N4534_RS03600 (N4534_03600) endA 743783..744490 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36112.28 Da        Isoelectric Point: 7.5355

>NTDB_id=731336 N4534_RS03570 WP_001055658.1 738965..739945(+) (pilT) [Salmonella enterica strain PNUSAS036471]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=731336 N4534_RS03570 WP_001055658.1 738965..739945(+) (pilT) [Salmonella enterica strain PNUSAS036471]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGTGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGTGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0R9N9F2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362