Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5980_RS11775 Genome accession   NZ_CP104651
Coordinates   2418681..2419718 (+) Length   345 a.a.
NCBI ID   WP_180012836.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain 2021CK-01005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2413681..2424718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5980_RS11765 (N5980_11755) - 2416730..2417836 (+) 1107 WP_261634966.1 acyltransferase family protein -
  N5980_RS11770 (N5980_11760) - 2417868..2418557 (-) 690 WP_261634967.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5980_RS11775 (N5980_11765) pilT 2418681..2419718 (+) 1038 WP_180012836.1 type IV pilus twitching motility protein PilT Machinery gene
  N5980_RS11780 (N5980_11770) pilU 2419747..2420868 (+) 1122 WP_004783234.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N5980_RS11785 (N5980_11775) fur 2420935..2421372 (-) 438 WP_004783237.1 ferric iron uptake transcriptional regulator -
  N5980_RS11790 (N5980_11780) - 2421485..2421883 (+) 399 WP_004783238.1 outer membrane protein assembly factor BamE -
  N5980_RS11795 (N5980_11785) - 2421987..2422316 (-) 330 WP_005221123.1 RnfH family protein -
  N5980_RS11800 (N5980_11790) - 2422316..2423395 (-) 1080 WP_261634968.1 hypothetical protein -
  N5980_RS11805 (N5980_11795) - 2423555..2424010 (-) 456 WP_004783243.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38279.86 Da        Isoelectric Point: 6.5056

>NTDB_id=731257 N5980_RS11775 WP_180012836.1 2418681..2419718(+) (pilT) [Acinetobacter variabilis strain 2021CK-01005]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICEYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSRGIISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=731257 N5980_RS11775 WP_180012836.1 2418681..2419718(+) (pilT) [Acinetobacter variabilis strain 2021CK-01005]
ATGGATATTACAGAACTGTTAGCCTTTTCTGCTAAAAATGGTGCGTCGGATTTACACTTATCAGCAGGTTTACCACCCAT
GATTCGTGTGGACGGGGAAGTACGCCGCATCAACCTGCCTGCACTAGAACATAAAGAAGTACACAAACTAGTGTACGACA
TCATGAATGACAAGCAACGCCGTGATTATGAAGAAAATCTGGAAACTGACTTCTCTTTTGAAGTTCCAGGCGTAGCCCGT
TTCCGTGTGAACGCATTTAACCAGAACCGCGGTGCAGGCGCAGTATTCCGTACCATTCCATCTAAAGTACTGACCATTGA
AGATCTAGGTATGGGCCAGATCTTTAAAGATATCTGTGAATATCCACGTGGTATTGTACTGGTGACAGGACCAACCGGTT
CGGGTAAATCAACGACGCTGGCGGCAATGCTGGACTATATCAATGACAACCGCTATGACCATATCCTGACAGTCGAAGAT
CCAATCGAATTTGTACACCAATCAAAAAAATGTCTGATCAACCAGCGTGAAGTACATCGTGATACGCATGGATTTAATGA
AGCATTACGTTCTGCATTACGTGAAGACCCGGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACCATCCGTCTGG
CACTCACTGCTGCGGAAACCGGTCACTTGGTCTTCGGTACCCTGCATACCACCTCAGCGGCAAAAACCATTGACCGTGTG
ATTGACGTGTTCCCTGCTGAAGAAAAAGACATGGTGCGTGCCATGTTGTCAGAATCGCTGCAAGCTGTTATTTCGCAAAC
CCTGTTGAAGAAAAATGGGGGGGGGCGTGTGGCTGCACACGAAATCATGATCGGTATTCCGGCGATCCGTAACCTGATCC
GTGAAAACAAAGTGGCACAGATGTACTCTGCGATTCAAACCGGTGCCAACTATGGCATGACCACGCTGGATCAAAGCCTG
AAAACGCTGGTATCGAGAGGTATTATCAGTCCGCAAGTCGCCCGTACTGCAGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.942

100

0.959
  pilT Acinetobacter baumannii D1279779

95.652

100

0.957
  pilT Acinetobacter baumannii strain A118

95.652

100

0.957
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Neisseria meningitidis 8013

68.986

100

0.69
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.684
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.181

96.232

0.675
  pilT Vibrio cholerae strain A1552

70.181

96.232

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.003

98.261

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.942

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388