Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N4230_RS03770 Genome accession   NZ_CP104482
Coordinates   786772..787752 (+) Length   326 a.a.
NCBI ID   WP_045901825.1    Uniprot ID   A0A5T3MD10
Organism   Salmonella enterica strain SalSpp_sample_08_No.4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 781772..792752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4230_RS03740 (N4230_03740) - 782349..783356 (+) 1008 WP_000252200.1 DUF1202 domain-containing protein -
  N4230_RS03745 (N4230_03745) hemW 783448..784584 (-) 1137 WP_080192308.1 radical SAM family heme chaperone HemW -
  N4230_RS03750 (N4230_03750) - 784577..785170 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  N4230_RS03755 (N4230_03755) yggU 785178..785468 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  N4230_RS03760 (N4230_03760) - 785465..786031 (-) 567 WP_001094848.1 YggT family protein -
  N4230_RS03765 (N4230_03765) - 786050..786754 (-) 705 WP_080192307.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4230_RS03770 (N4230_03770) pilT 786772..787752 (+) 981 WP_045901825.1 type IV pilus twitching motility protein PilT Machinery gene
  N4230_RS03775 (N4230_03775) - 787884..788570 (+) 687 WP_093984170.1 IclR family transcriptional regulator -
  N4230_RS03780 (N4230_03780) ruvX 788617..789033 (-) 417 WP_001285490.1 Holliday junction resolvase RuvX -
  N4230_RS03785 (N4230_03785) - 789033..789596 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  N4230_RS03790 (N4230_03790) gshB 789812..790759 (-) 948 WP_000593248.1 glutathione synthase -
  N4230_RS03795 (N4230_03795) rsmE 790779..791510 (-) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N4230_RS03800 (N4230_03800) endA 791587..792294 (-) 708 WP_000286133.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36137.38 Da        Isoelectric Point: 8.0973

>NTDB_id=729929 N4230_RS03770 WP_045901825.1 786772..787752(+) (pilT) [Salmonella enterica strain SalSpp_sample_08_No.4]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAKIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=729929 N4230_RS03770 WP_045901825.1 786772..787752(+) (pilT) [Salmonella enterica strain SalSpp_sample_08_No.4]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGAAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTACGTGAAGATCCTGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5T3MD10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362