Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N4P55_RS26820 Genome accession   NZ_CP104408
Coordinates   5861600..5862634 (-) Length   344 a.a.
NCBI ID   WP_064454547.1    Uniprot ID   A0A172Z8L4
Organism   Pseudomonas fluorescens strain Ant01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5856600..5867634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4P55_RS26790 (N4P55_26805) ruvX 5856687..5857124 (+) 438 WP_003213744.1 Holliday junction resolvase RuvX -
  N4P55_RS26795 (N4P55_26810) pyrR 5857208..5857714 (+) 507 WP_064454543.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  N4P55_RS26800 (N4P55_26815) - 5857739..5858743 (+) 1005 WP_259763341.1 aspartate carbamoyltransferase catalytic subunit -
  N4P55_RS26805 (N4P55_26820) - 5858740..5860011 (+) 1272 WP_064454545.1 dihydroorotase -
  N4P55_RS26810 (N4P55_26825) - 5860146..5860574 (-) 429 WP_064454546.1 NINE protein -
  N4P55_RS26815 (N4P55_26830) - 5860824..5861459 (+) 636 WP_053258477.1 C40 family peptidase -
  N4P55_RS26820 (N4P55_26835) pilT 5861600..5862634 (-) 1035 WP_064454547.1 type IV pilus twitching motility protein PilT Machinery gene
  N4P55_RS26825 (N4P55_26840) - 5862692..5863387 (+) 696 WP_064454548.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4P55_RS26830 (N4P55_26845) proC 5863412..5864230 (+) 819 WP_064454549.1 pyrroline-5-carboxylate reductase -
  N4P55_RS26835 (N4P55_26850) - 5864270..5864857 (+) 588 WP_053258481.1 YggT family protein -
  N4P55_RS26840 (N4P55_26855) - 5865049..5866188 (+) 1140 WP_262666477.1 homoserine O-succinyltransferase MetX -
  N4P55_RS26845 (N4P55_26860) metW 5866196..5866816 (+) 621 WP_003213768.1 methionine biosynthesis protein MetW -
  N4P55_RS26850 (N4P55_26865) - 5866838..5867272 (+) 435 WP_064454551.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37333.00 Da        Isoelectric Point: 6.7811

>NTDB_id=729684 N4P55_RS26820 WP_064454547.1 5861600..5862634(-) (pilT) [Pseudomonas fluorescens strain Ant01]
MDITELLTASVRRGASDLHLSAGVAPMLRVDGEVWPLDGPALSPPQVADLLSPLLNQYQEKDFETSLETDFAFELPGVAR
FRANVFQQARGMGAVFRTIATEVQSLESLGLGEVFQRIARFPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKMALINQRQVQRDTHSFSTALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTVHTTSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVSEGLVSREEARGQARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=729684 N4P55_RS26820 WP_064454547.1 5861600..5862634(-) (pilT) [Pseudomonas fluorescens strain Ant01]
ATGGATATCACTGAATTGCTGACGGCCAGCGTGCGTCGTGGCGCCTCTGACCTGCATTTGTCGGCTGGCGTGGCGCCGAT
GCTGCGTGTGGATGGCGAGGTCTGGCCCTTGGACGGCCCGGCACTGTCCCCCCCGCAAGTGGCGGATTTATTGAGCCCTT
TGCTCAACCAATACCAAGAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGCTGCCGGGCGTGGCACGG
TTCCGGGCGAATGTGTTCCAGCAGGCGCGCGGCATGGGCGCGGTGTTTCGTACCATTGCGACCGAAGTACAAAGCCTGGA
AAGCCTCGGGCTGGGAGAAGTGTTCCAGCGGATCGCCCGGTTTCCGCGCGGCCTGGTGTTGGTGACCGGGCCAACCGGGT
CCGGCAAGTCCACGACGCTGGCAGCGATGATCGACCATCTTAATCAACACCGGCGCCAGCACATTCTTACGCTGGAAGAC
CCCATCGAATTTATCCACAGGCCGAAAATGGCCTTGATCAACCAGCGGCAGGTACAACGCGATACACACAGTTTTTCCAC
GGCACTGCGCTCGGCGCTACGGGAAGACCCGGATGTCATTCTGGTGGGTGAGTTGCGCGACCTGGAAACCATACGCCTGG
CGCTGACGGCGGCTGAGACCGGCCACCTGGTATTTGGCACCGTGCACACCACTTCGGCGGCAAAGACCGTGGACAGGCTG
GTGGATGTGTTCCCGGCGGGGGAAAAGGCCATGGTTCGCTCGATGCTGTCGGAGTCGTTGCAGGCCGTGGTGTCCCAAGT
GCTGGTCAAGAAGATCGGCGGCGGGCGGGTGGCGGCTCACGAAATCATGCTGGGCACGCCGGCCATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAAATGGTCTCCGCGATACAGACCGGTGGGGCGCTGGGCATGAAAACGTTGGATATGAGCTTG
AAGGCGTTGGTCAGCGAAGGGTTGGTCAGCCGCGAGGAGGCGCGGGGGCAGGCGAGGGTGCCGGGGGATATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(83-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172Z8L4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.727

99.128

0.721
  pilT Pseudomonas stutzeri DSM 10701

70.674

99.128

0.701
  pilT Acinetobacter nosocomialis M2

69.501

99.128

0.689
  pilT Acinetobacter baumannii D1279779

69.208

99.128

0.686
  pilT Acinetobacter baumannii strain A118

69.208

99.128

0.686
  pilT Acinetobacter baylyi ADP1

68.622

99.128

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.688

97.965

0.634
  pilT Vibrio cholerae strain A1552

64.688

97.965

0.634
  pilT Legionella pneumophila strain Lp02

63.018

98.256

0.619
  pilT Legionella pneumophila strain ERS1305867

63.018

98.256

0.619
  pilT Neisseria meningitidis 8013

61.176

98.837

0.605
  pilT Neisseria gonorrhoeae MS11

60.882

98.837

0.602
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.254

97.384

0.48
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392
  pilU Vibrio cholerae strain A1552

39.13

100

0.392