Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FOY96_RS03440 Genome accession   NZ_AP019632
Coordinates   714709..715689 (+) Length   326 a.a.
NCBI ID   WP_143346508.1    Uniprot ID   -
Organism   Enterobacter asburiae strain 1808-013     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 709709..720689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOY96_RS03400 (EAS1808013_006590) - 709790..710116 (+) 327 WP_014885198.1 YggL family protein -
  FOY96_RS03405 (EAS1808013_006600) - 710173..710889 (+) 717 WP_143346505.1 DUF2884 domain-containing protein -
  FOY96_RS03410 (EAS1808013_006610) - 710951..711313 (-) 363 WP_071993605.1 endonuclease domain-containing protein -
  FOY96_RS03415 (EAS1808013_006620) hemW 711383..712519 (-) 1137 WP_143346506.1 radical SAM family heme chaperone HemW -
  FOY96_RS03420 (EAS1808013_006630) - 712512..713105 (-) 594 WP_045403921.1 XTP/dITP diphosphatase -
  FOY96_RS03425 (EAS1808013_006640) yggU 713109..713405 (-) 297 WP_023309131.1 DUF167 family protein YggU -
  FOY96_RS03430 (EAS1808013_006650) - 713402..713968 (-) 567 WP_045888331.1 YggT family protein -
  FOY96_RS03435 (EAS1808013_006660) - 713990..714691 (-) 702 WP_143346507.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOY96_RS03440 (EAS1808013_006670) pilT 714709..715689 (+) 981 WP_143346508.1 type IV pilus twitching motility protein PilT Machinery gene
  FOY96_RS03445 (EAS1808013_006680) ruvX 715700..716116 (-) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  FOY96_RS03450 (EAS1808013_006690) - 716116..716676 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  FOY96_RS03455 (EAS1808013_006700) gshB 716764..717711 (-) 948 WP_029739222.1 glutathione synthase -
  FOY96_RS03460 (EAS1808013_006710) rsmE 717731..718462 (-) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FOY96_RS03465 (EAS1808013_006720) endA 718514..719221 (-) 708 WP_023333343.1 deoxyribonuclease I -
  FOY96_RS03470 (EAS1808013_006730) - 719316..719813 (-) 498 WP_143346509.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35505.57 Da        Isoelectric Point: 6.9108

>NTDB_id=72952 FOY96_RS03440 WP_143346508.1 714709..715689(+) (pilT) [Enterobacter asburiae strain 1808-013]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFQPPDVGALLKAWLNDEQQGIWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLASLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQGLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=72952 FOY96_RS03440 WP_143346508.1 714709..715689(+) (pilT) [Enterobacter asburiae strain 1808-013]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGGATATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTTTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAGACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGGGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Pseudomonas aeruginosa PAK

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

98.773

0.436
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment