Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N4267_RS11630 Genome accession   NZ_CP104354
Coordinates   2575241..2576347 (-) Length   368 a.a.
NCBI ID   WP_113601003.1    Uniprot ID   -
Organism   Vibrio cholerae strain PNUSAV001140     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2570241..2581347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4267_RS11605 (N4267_11605) gshB 2570855..2571811 (+) 957 WP_000593310.1 glutathione synthase -
  N4267_RS11610 (N4267_11610) - 2571847..2572410 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  N4267_RS11615 (N4267_11615) ruvX 2572477..2572899 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  N4267_RS11620 (N4267_11620) tyrS 2572937..2574217 (-) 1281 WP_147422776.1 tyrosine--tRNA ligase -
  N4267_RS11625 (N4267_11625) - 2574326..2575123 (-) 798 WP_162806856.1 helix-turn-helix transcriptional regulator -
  N4267_RS11630 (N4267_11630) pilU 2575241..2576347 (-) 1107 WP_113601003.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N4267_RS11635 (N4267_11635) pilT 2576362..2577399 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  N4267_RS11640 (N4267_11640) - 2577425..2578135 (+) 711 WP_001256648.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4267_RS11645 (N4267_11645) proC 2578188..2579006 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  N4267_RS11650 (N4267_11650) - 2579060..2579617 (+) 558 WP_001087261.1 YggT family protein -
  N4267_RS11655 (N4267_11655) yggU 2579617..2579907 (+) 291 WP_001914289.1 DUF167 family protein YggU -
  N4267_RS11660 (N4267_11660) - 2579952..2580383 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  N4267_RS11665 (N4267_11665) - 2580453..2581055 (+) 603 WP_055028323.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41327.30 Da        Isoelectric Point: 6.5801

>NTDB_id=729018 N4267_RS11630 WP_113601003.1 2575241..2576347(-) (pilU) [Vibrio cholerae strain PNUSAV001140]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLQAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEILLNSPRISDLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=729018 N4267_RS11630 WP_113601003.1 2575241..2576347(-) (pilU) [Vibrio cholerae strain PNUSAV001140]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCGCAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCAGGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAGGCGAATTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAGCT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCAATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGCGAAATTCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGGCATTTGTGTATGGCCACGCTGCACGCTAACAATGCCAACCAAGCGTTAGAGCGGATTTTGCAT
CTGGTGCCCAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCTCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAATTCTGCTCAATAGCCCGCGCATTTCCGATTTGATCCGCC
GTGGCGAGCTACACGAGCTAAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCTTTGTAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCTAACGATCTGCGTCTGATGCT
CAAAACCAAGCGTGGTGATAACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

98.913

100

0.989
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain ERS1305867

43.068

92.12

0.397
  pilT Legionella pneumophila strain Lp02

43.068

92.12

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.129

84.239

0.389
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Vibrio cholerae strain A1552

42.515

90.761

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

90.761

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.298

92.12

0.38
  pilT Acinetobacter baylyi ADP1

42.769

88.315

0.378
  pilT Neisseria meningitidis 8013

38.841

93.75

0.364
  pilT Neisseria gonorrhoeae MS11

38.617

94.293

0.364