Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P1J23_RS04200 Genome accession   NZ_CP119740
Coordinates   852224..853204 (+) Length   326 a.a.
NCBI ID   WP_072004315.1    Uniprot ID   -
Organism   Escherichia coli strain E94     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 847224..858204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P1J23_RS04170 (P1J23_04170) yggM 847739..848746 (+) 1008 WP_000745217.1 DUF1202 family protein -
  P1J23_RS04175 (P1J23_04175) hemW 848901..850037 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  P1J23_RS04180 (P1J23_04180) rdgB 850030..850623 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  P1J23_RS04185 (P1J23_04185) yggU 850631..850921 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  P1J23_RS04190 (P1J23_04190) yggT 850918..851484 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  P1J23_RS04195 (P1J23_04195) yggS 851502..852206 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  P1J23_RS04200 (P1J23_04200) pilT 852224..853204 (+) 981 WP_072004315.1 type IV pilus twitching motility protein PilT Machinery gene
  P1J23_RS04205 (P1J23_04205) ruvX 853367..853783 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  P1J23_RS04210 (P1J23_04210) yqgE 853783..854346 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  P1J23_RS04215 (P1J23_04215) gshB 854455..855405 (-) 951 WP_000593273.1 glutathione synthase -
  P1J23_RS04220 (P1J23_04220) rsmE 855418..856149 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  P1J23_RS04225 (P1J23_04225) endA 856229..856936 (-) 708 WP_000286500.1 deoxyribonuclease I -
  P1J23_RS04230 (P1J23_04230) yggI 857031..857528 (-) 498 WP_276171153.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35933.11 Da        Isoelectric Point: 5.5860

>NTDB_id=728004 P1J23_RS04200 WP_072004315.1 852224..853204(+) (pilT) [Escherichia coli strain E94]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHYMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=728004 P1J23_RS04200 WP_072004315.1 852224..853204(+) (pilT) [Escherichia coli strain E94]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTATATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTAACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGATGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362