Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NYP54_RS08985 Genome accession   NZ_CP103983
Coordinates   2062287..2063327 (-) Length   346 a.a.
NCBI ID   WP_283156218.1    Uniprot ID   -
Organism   Cysteiniphilum sp. QT6929     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2057287..2068327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYP54_RS08960 (NYP54_08960) - 2057434..2057703 (+) 270 WP_283156214.1 type II toxin-antitoxin system RelE/ParE family toxin -
  NYP54_RS08965 (NYP54_08965) asnS 2057797..2059200 (-) 1404 WP_283156215.1 asparagine--tRNA ligase -
  NYP54_RS08970 (NYP54_08970) glyQ 2059674..2060561 (+) 888 WP_151192831.1 glycine--tRNA ligase subunit alpha -
  NYP54_RS08975 (NYP54_08975) - 2060558..2060743 (-) 186 WP_283156216.1 CBU_0585 family protein -
  NYP54_RS08980 (NYP54_08980) - 2060835..2062286 (+) 1452 WP_283156217.1 potassium transporter TrkG -
  NYP54_RS08985 (NYP54_08985) pilT 2062287..2063327 (-) 1041 WP_283156218.1 type IV pilus twitching motility protein PilT Machinery gene
  NYP54_RS08990 (NYP54_08990) - 2063374..2064072 (+) 699 WP_283156219.1 YggS family pyridoxal phosphate-dependent enzyme -
  NYP54_RS08995 (NYP54_08995) - 2064781..2065809 (+) 1029 WP_283156220.1 ABC transporter substrate-binding protein -
  NYP54_RS09000 (NYP54_09000) - 2065987..2066199 (+) 213 WP_117003826.1 hypothetical protein -
  NYP54_RS09005 (NYP54_09005) - 2066221..2066523 (-) 303 Protein_1745 ferritin-like domain-containing protein -
  NYP54_RS09010 (NYP54_09010) - 2066704..2067882 (+) 1179 WP_283155794.1 IS3 family transposase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 39002.61 Da        Isoelectric Point: 6.3904

>NTDB_id=726774 NYP54_RS08985 WP_283156218.1 2062287..2063327(-) (pilT) [Cysteiniphilum sp. QT6929]
MNLTELLSLCISKKASDLHLSSGAKAKLRIDGHIESVSDELLTEETLENYLHEVMSSEQYQHFQETLEYDFSVHLPELHS
RFRVNAFKQDRGSALVFRVIPESVYTLDELEAPDIFKELMHKPHGLIVVTGPTGSGKSTTLAAMIDHINKTEKGHIISIE
DPIEFTHKSQQCLINQREVKRDTLSFNAALRAALREDPDYILVGEMRDVETIRLALTAAETGHLVFATLHTSSAPKTIDR
IINVFPGDEQPMIRSMLSESLQAVIAQRLIRKKEGGRVAAHEVLIANTAVRNLIRENKIPQLYNVLQTSQNKGMQTLEQA
LTKLFNRGIIDAEELKKYNTIQQPMS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=726774 NYP54_RS08985 WP_283156218.1 2062287..2063327(-) (pilT) [Cysteiniphilum sp. QT6929]
ATGAACCTTACGGAATTATTATCGCTTTGTATTAGCAAAAAGGCATCGGATTTACATCTCTCTAGTGGCGCTAAAGCCAA
ACTGCGCATTGATGGACATATTGAATCTGTCAGTGATGAGCTATTAACTGAGGAAACCCTTGAGAACTATTTACATGAAG
TCATGAGCTCAGAGCAGTATCAACATTTTCAAGAAACCTTAGAATATGATTTCTCTGTCCATTTGCCAGAGCTTCATTCG
CGTTTTAGGGTCAATGCCTTTAAACAAGATCGCGGCAGTGCGCTTGTTTTTCGCGTGATCCCTGAAAGTGTTTATACATT
GGATGAGCTTGAAGCACCTGATATTTTCAAAGAATTGATGCATAAACCACATGGACTGATTGTCGTCACCGGTCCCACAG
GCAGTGGTAAGAGTACGACACTTGCAGCGATGATTGATCATATTAACAAAACCGAAAAAGGGCATATTATCTCGATTGAA
GACCCTATTGAGTTTACCCATAAATCACAGCAATGCCTCATCAATCAACGTGAGGTTAAACGTGATACTTTAAGCTTTAA
TGCAGCTCTACGTGCGGCACTGCGCGAGGATCCAGATTATATTCTGGTTGGCGAGATGCGTGATGTTGAGACCATTCGTC
TGGCGTTAACCGCCGCTGAAACGGGGCACTTGGTATTTGCCACCTTGCATACCTCATCAGCACCTAAAACGATTGATCGG
ATTATCAATGTCTTTCCAGGGGACGAGCAGCCAATGATCCGCTCGATGCTCTCAGAGTCTTTACAAGCCGTTATTGCTCA
GCGACTTATTCGTAAAAAAGAAGGCGGACGCGTTGCAGCTCATGAAGTCCTCATTGCGAATACCGCCGTGCGCAATCTTA
TTCGTGAGAATAAAATTCCTCAGCTTTATAATGTGTTGCAGACCAGTCAAAATAAGGGCATGCAAACCTTAGAACAAGCA
CTCACTAAACTATTTAATCGAGGAATTATCGATGCTGAAGAGCTTAAAAAGTACAACACCATACAACAACCGATGTCCTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

56.772

100

0.569
  pilT Neisseria gonorrhoeae MS11

56.484

100

0.566
  pilT Acinetobacter baumannii D1279779

55.78

100

0.558
  pilT Acinetobacter baumannii strain A118

55.78

100

0.558
  pilT Acinetobacter nosocomialis M2

55.78

100

0.558
  pilT Acinetobacter baylyi ADP1

57.704

95.665

0.552
  pilT Pseudomonas aeruginosa PAK

55.685

99.133

0.552
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.548

97.11

0.549
  pilT Vibrio cholerae strain A1552

56.548

97.11

0.549
  pilT Legionella pneumophila strain ERS1305867

57.273

95.376

0.546
  pilT Legionella pneumophila strain Lp02

57.273

95.376

0.546
  pilT Pseudomonas stutzeri DSM 10701

55.786

97.399

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.849

95.665

0.477
  pilU Acinetobacter baylyi ADP1

43.731

94.509

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.821

0.408
  pilU Vibrio cholerae strain A1552

40.308

93.931

0.379