Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NYP20_RS27550 Genome accession   NZ_CP103965
Coordinates   6237909..6238943 (-) Length   344 a.a.
NCBI ID   WP_259497272.1    Uniprot ID   -
Organism   Pseudomonas sp. N3-W     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6232909..6243943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYP20_RS27520 (NYP20_27520) ruvX 6232975..6233412 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  NYP20_RS27525 (NYP20_27525) pyrR 6233503..6234009 (+) 507 WP_259497268.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NYP20_RS27530 (NYP20_27530) - 6234037..6235041 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  NYP20_RS27535 (NYP20_27535) - 6235038..6236309 (+) 1272 WP_259497269.1 dihydroorotase -
  NYP20_RS27540 (NYP20_27540) - 6236393..6236824 (-) 432 WP_259497270.1 TM2 domain-containing protein -
  NYP20_RS27545 (NYP20_27545) - 6237075..6237701 (+) 627 WP_259497271.1 C40 family peptidase -
  NYP20_RS27550 (NYP20_27550) pilT 6237909..6238943 (-) 1035 WP_259497272.1 type IV pilus twitching motility protein PilT Machinery gene
  NYP20_RS27555 (NYP20_27555) - 6239000..6239680 (+) 681 WP_259497273.1 YggS family pyridoxal phosphate-dependent enzyme -
  NYP20_RS27560 (NYP20_27560) proC 6239727..6240545 (+) 819 WP_259497274.1 pyrroline-5-carboxylate reductase -
  NYP20_RS27565 (NYP20_27565) - 6240570..6241157 (+) 588 WP_259497275.1 YggT family protein -
  NYP20_RS27570 (NYP20_27570) - 6241157..6241453 (+) 297 WP_259497276.1 DUF167 domain-containing protein -
  NYP20_RS27575 (NYP20_27575) - 6241637..6242776 (+) 1140 WP_259497277.1 homoserine O-acetyltransferase -
  NYP20_RS27580 (NYP20_27580) metW 6242784..6243404 (+) 621 WP_259497278.1 methionine biosynthesis protein MetW -
  NYP20_RS27585 (NYP20_27585) - 6243431..6243865 (+) 435 WP_259497279.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38074.70 Da        Isoelectric Point: 6.9778

>NTDB_id=726687 NYP20_RS27550 WP_259497272.1 6237909..6238943(-) (pilT) [Pseudomonas sp. N3-W]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGDVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNNNRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KELVAKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=726687 NYP20_RS27550 WP_259497272.1 6237909..6238943(-) (pilT) [Pseudomonas sp. N3-W]
ATGGATATCACTGAACTGTTGGCATTCAGCGCCAAACAGGGGGCGTCCGACCTGCACCTGTCTGCCGGCCTGCCACCGAT
GATCCGCGTCGATGGCGATGTGCGACGCATCAACCTGCCGGCCCTGGATCACAAACAGGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGATTTCGAGAAGCATCTGGAAACCGACTTTTCCTTTGAAGTGCCTGGCGTGGCGCGC
TTTCGGGTTAACGCCTTCAACCAGAATCGCGGTGCTGGCGCGGTGTTCCGCACCATTCCCTCGAAAGTCCTGAGCATGGA
CGACTTGGGCATGGGCGATGTGTTTCGCAAGATAACCGAGGCGCCACGCGGCCTGGTACTGGTGACGGGGCCGACCGGGT
CCGGCAAGTCCACGACCCTGGCGGCGATGATCGATTATCTGAACAACAACCGCCATCACCATATTCTCACCATCGAAGAC
CCAATCGAATTCGTTCACGAATCGCGCAAATGCCTGATCAACCAGCGCGAAGTCCATCGCGATACCCGCAGCTTCGCCAC
CGCTCTGCGTTCGGCGTTGCGCGAGGACCCGGACGTGATTCTGGTGGGGGAGATGCGTGACCTGGAAACCATTCGCCTGG
CCCTGACTGCCGCCGAAACCGGACACCTGGTGTTTGGCACGCTGCACACTACCTCGGCGGCGAAAACCATCGACCGGGTG
GTGGACGTGTTTCCGGGTGATGAGAAGTCGATGGTGCGTTCGATGCTTTCAGAGTCGCTGCTGGCGGTGGTGTCCCAGAC
CCTGGTCAAGAAGATCGGCGGCGGCCGCATCGCGGCGCACGAGATCATGCTCGGCACCTCGGCGATCCGTAACCTGATCC
GTGAAGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGCGGGAATCTGGGGATGCAGACGCTGGATATGTGTTTG
AAGGAGTTGGTGGCCAAGGGCTTGATCAGCCGCGAGCATGCGCGGGAGAAGGCGCGAACGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Pseudomonas aeruginosa PAK

86.919

100

0.869
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.13

100

0.392