Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PWR57_RS20105 Genome accession   NZ_CP119441
Coordinates   3987432..3988412 (-) Length   326 a.a.
NCBI ID   WP_052234598.1    Uniprot ID   -
Organism   Escherichia coli O121 strain 201805972     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3982432..3993412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWR57_RS20075 (PWR57_20045) yggI 3983087..3983584 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  PWR57_RS20080 (PWR57_20050) endA 3983679..3984386 (+) 708 WP_000286500.1 deoxyribonuclease I -
  PWR57_RS20085 (PWR57_20055) rsmE 3984466..3985197 (+) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PWR57_RS20090 (PWR57_20060) gshB 3985210..3986160 (+) 951 WP_000593273.1 glutathione synthase -
  PWR57_RS20095 (PWR57_20065) yqgE 3986269..3986832 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  PWR57_RS20100 (PWR57_20070) ruvX 3986832..3987248 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  PWR57_RS20105 (PWR57_20075) pilT 3987432..3988412 (-) 981 WP_052234598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PWR57_RS20110 (PWR57_20080) yggS 3988430..3989134 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  PWR57_RS20115 (PWR57_20085) yggT 3989152..3989718 (+) 567 WP_073860367.1 osmotic shock tolerance protein YggT -
  PWR57_RS20120 (PWR57_20090) yggU 3989715..3990005 (+) 291 WP_000994920.1 DUF167 family protein YggU -
  PWR57_RS20125 (PWR57_20095) rdgB 3990013..3990606 (+) 594 WP_001174777.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  PWR57_RS20130 (PWR57_20100) hemW 3990599..3991735 (+) 1137 WP_000239943.1 radical SAM family heme chaperone HemW -
  PWR57_RS20135 (PWR57_20105) yggM 3991890..3992897 (-) 1008 WP_000745244.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36024.24 Da        Isoelectric Point: 6.0804

>NTDB_id=726485 PWR57_RS20105 WP_052234598.1 3987432..3988412(-) (pilT) [Escherichia coli O121 strain 201805972]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGAMGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=726485 PWR57_RS20105 WP_052234598.1 3987432..3988412(-) (pilT) [Escherichia coli O121 strain 201805972]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGATGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.597

97.546

0.445
  pilT Legionella pneumophila strain Lp02

45.597

97.546

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.253

100

0.39
  pilU Acinetobacter baylyi ADP1

36.061

100

0.365